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低氧对NG 108-15细胞生长及分化的影响

顾锦法, 施玉liang

中科院上海生理研究所. 上海(200031)

摘要

低氧明显降低NG108-15末分化细胞增殖和存活率, 使分化完成的细胞大量死亡; 低氧影响NG108-15细胞的分化, 使细胞在分化中出现体积膨大, 突起短等异常特征, 经焦油紫染色, 胞质中无尼氏体(即不着色)的细胞增多。图1表2参10

关键词： NG108－15细胞; 低氧; 细胞培养; 生长; 分化

Effect of hypoxia on the growth and differentiation of NG 108-15 cells

Gu Jinfa, Shi Yuliang

Shanghai Institute of Physiology, Chinese Academy of Sciences. Shanghai

Abstract

The neuroblastoma x glioma hybrid clone NG108-15 which were derived by fusion of mouse neuroblastoma clone N18TG6 with rat glioma clone C6BU1 prolifenates in the presence of fetal calf serum, but differentiates when intracellular cAMP concen tration is made to increase by addition of dBcAMP to the cultural medium. After differentiation, the cells express many neural properties. In this experiment, by means of automatic colorimetric microassay for quantifying the growth and Brilliant Cresyliolett stain for revealing Nissl substance, the effect of hypoxia(2% I_(2))on the hybrid cells was investigated. Hypoxia depressed proliferation of non-differentiated cells and induced death of differentiated ones. Hypoxia inhibited differentiation, either non-differentiated or differentiating cells, in the direction to be a neuro-like cell: some cells became large flat with short processes and had no Nissl substance. Whether hypoxia would enable non-differentiated NG108-15 cells to differentiate in a direction to express more properties of glial cell is an interesting problem.

Key words: G108-15cell;Hypoxia;Cell culture;Growth;Differentiation

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引用本文：

顾锦法, 施玉liang. 低氧对NG 108-15细胞生长及分化的影响[J]. 生理学报 1994; 46 (3): .

Gu Jinfa, Shi Yuliang. Effect of hypoxia on the growth and differentiation of NG 108-15 cells. Acta Physiol Sin 1994; 46 (3): (in Chinese with English abstract).