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嗜热蓝藻优雅粘囊藻藻胆体的特性和别藻蓝蛋白的亚基组成

赵东旭, 曾繁杰, 容寿榆, 方昭希

北京大学生物系. 北京(100871)；中科院海洋研究所. 山东,青岛(266071)

摘要

从聚丙烯酰胺凝胶电泳(PAGE)、吸收光谱和二阶导数图谱可证明, 优雅粘囊藻藻胆体(PBS) 含有一种红色藻胆蛋白, 两种C-藻蓝蛋白和三种别藻蓝蛋白。但是用PAGE和羟基磷灰石柱 层析分离和纯化所得藻胆蛋白, 一般仅得到一种C-PC和一种亚基组成特殊的别藻蓝蛋白APC。该APC吸收光谱和荧光发射相同于已报告的APC660nm。但其亚基组成是(#alpha# #alpha#^#beta# #beta#^)_(2)而不是(#alpha#^#alpha#_(2)#beta#_(2)#beta#^)Lc~(10)或(#alpha# #beta#)_(3)。图8参8

关键词： 优雅粘囊藻; 藻胆体; 别藻蓝蛋白; 亚基组成; 嗜热蓝藻

Characterization of phycobilisome and subunit composition of allophycyanin-APC 660 nm from the thermophilic cyanobacterium Myxosarcina concinna Printz

Zhao Dongxu, Zeng Fanjie, Rong Shouyu, Fang Zhaoxi

Department of Biology, Peking University. Beijing；China(266071)

Abstract

Three allophycocyanins, two phycocyanins and one kind of red protein was separated from the intact PBS by PAA 4%~30% gradient gel electrophoresis. Only one easily dissociable C-phycoyanin and one relatively stable allophycocyanin (APC 660 nm) were obtained by using preparative PAGE and hydroxylapatite column chromatography. The fluorescence emission spectrum of the APC 660 nm at room temperature exhibited the maximum at 660nm and its fluorescence life time was shown to be 2.063ns. The molecular weights of the APC 660nm were found to be 140kD and 147kD, The APC 660nm contained two light and two heavy subunits having molecular weights of 16000(#alpha#), 17 000(#alpha#^), 18 400(#beta#), and 19 000(#beta#^), respectively. The subunit composition of APC 660 nm deduced from these results would be (#alpha##alpha#^ #beta##beta#^)_(2), which was different from the complex(#alpha#^ #alpha#_(2)#beta#_(2)#beta#^)Lc~(10) for APC 664 nm from thermophilic cyanobacterium Mastigocladus laminosus and (#alpha##beta#)_(3)for APC 660 nm from common blue-green algae.

Key words: Phycobilisoine;Allophycocyanin;Subunit composition;Myxosarcina concinna; Thermophilic cyanobacterium

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引用本文：

赵东旭, 曾繁杰, 容寿榆, 方昭希. 嗜热蓝藻优雅粘囊藻藻胆体的特性和别藻蓝蛋白的亚基组成[J]. 生理学报 1994; 46 (3): .

Zhao Dongxu, Zeng Fanjie, Rong Shouyu, Fang Zhaoxi. Characterization of phycobilisome and subunit composition of allophycyanin-APC 660 nm from the thermophilic cyanobacterium Myxosarcina concinna Printz. Acta Physiol Sin 1994; 46 (3): (in Chinese with English abstract).