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绿豆上胚轴中油菜素甾酮的结合特性

徐如涓, 何宇炯, 王玉琴, 赵毓橘

中科院上海植物生理研究所. 上海(200032)

摘要

绿豆上胚轴中存在油菜素甾酮的结合位点, 在10 000×g沉淀组份中结合活性较高; 4℃保温1h, ~(125)I-油菜素甾酮与组分的结合逐步趋于饱和; pH7.5时, 结合活性最高; 胰蛋白酶浓度升高, 结合活性下降; 相似结构的甾体类化合物有部分竞争现象。图5表2参13(S5)

关键词： 油菜素甾酮; 绿豆; 上胚轴; 结合位点

Preliminary study of brassinosterone binding sites from mung bean epicotyls

Xu Rujuan, He Yujiong, Wang Yuqin, Zhao Yuju

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai

Abstract

Different fractions of mung bean epicotyl extract were tested for brassinosterone binding. The 10 000×g fraction showed stronger brassinosterone binding than other fractions (Table 1). The brassinosterone binding to 10 000×g fraction was saturated after a 1 h incubation (Fig.1). The binding was temperature-dependent, showing a maximum at 4℃ (Fig.2). The amount of brassinosterone bound is dependent on pH, the optimal pH being 7.5(Fig.3). Trypsin treatment weakened the brassinosterone binding (Fig.4). This result indicates that proteins may be involved in the brassinosterone-binding site. Several analogues of brassinosterone could also bind to the brassinosterone-binding site to various extents(Table 2).

Key words: Brassinosterone;Mung bean;Epicotyl;Binding site

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引用本文：

徐如涓, 何宇炯, 王玉琴, 赵毓橘. 绿豆上胚轴中油菜素甾酮的结合特性[J]. 生理学报 1994; 46 (3): .

Xu Rujuan, He Yujiong, Wang Yuqin, Zhao Yuju. Preliminary study of brassinosterone binding sites from mung bean epicotyls. Acta Physiol Sin 1994; 46 (3): (in Chinese with English abstract).