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钙调素对花粉萌发和花粉管生长的效应

龚明, 杨中汉, 曹宗巽

北京大学生物系. 北京(100871)

摘要

牛脑和玉米胚CaM能显著促进花粉萌发和花粉管生长, 而CaM抑制剂TEP、CPZ及另外两个专一性更强的抑制剂Compound 48/80和W_(7)均严重抑制甚至阻止花粉的萌发。用对CaM亲和性较低的W_(7)同系物W_(5), 在与W_(7)同样浓度下, 对花粉萌发和花粉管生 长无明显影响。此外, W_(7)对花粉萌发和花粉管生长的抑制效应可被外源CaM所消除。在花粉萌发过程中, 其内源CaM含量显著上升, 在花粉萌发率接近最大值时, 花粉CaM含量达最高水平。图5参20

关键词： 钙调素; 钙调素抑制剂; 钙调素含量; 花粉萌发

Involvement of calmodulin in pollen germination and pollen tube growth

Gong Ming, Yang Zhonghan, Cao Zongxun(Tsao T H)

Department of Biology, Peking University. Beijing

Abstract

Calmodulin from both bovine brain and maize germs significantly promoted pollen germination and tube growth of Pinus yunnanensis and Nicotiana tabacum; CaM antagonists TFP and CPZ, and two other more specific drugs, W_(7) and compound 48/80, inhibited or stopped pollen germination and tube growth; while W_(5), a less active analogue of W_(7), had little effect. The inhibitory effects of W_(7)could be abolished partially or totally by exogenous CaM. In addition, the CaM content in pine pollen increased significantly during pollen germination and reached a maximum when the percentage of germinated pollen was near its maximum.

Key words: Calmodulin;CaM antagonists;CaM content;Pollen germination

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引用本文：

龚明, 杨中汉, 曹宗巽. 钙调素对花粉萌发和花粉管生长的效应[J]. 生理学报 1994; 46 (3): .

Gong Ming, Yang Zhonghan, Cao Zongxun(Tsao T H). Involvement of calmodulin in pollen germination and pollen tube growth. Acta Physiol Sin 1994; 46 (3): (in Chinese with English abstract).