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大鼠脑内脑钠素mRNA分布的原位杂交

刘兢, 马咏, 李菁, 张爱民, 刘宏, 徐洵

中国科学技术大学生物系. 安徽,合肥(230026)

摘要

大鼠脑中存在脑钠肽基因的表达, 其中下丘脑及丘脑的边缘部分脑钠肽的mRNA浓度最高, 杏仁核簇中浓度较高, 嗅区其次, 海马回和大脑皮层中浓度较低, 小脑和延髓中几乎没有基因表达。

关键词： 脑钠肽; 原位杂交; 鼠脑; 信使核糖核酸

Detection of messenger RNA coding for BNP precursor in rat brain by in situ hybridization

Liu Jing, Ma Yong, Li Jing, Zhang Aimin, Liu Hong, Xu Xun

Department of Biology, University of Science and Technology of China. Hefei,Anhui

Abstract

Messenger RNA coding for BNP precursor has been detected and mapped in histological sections of rat brain by in situ hybridization using specific rat BNPDNa probes labelled with ~(32)P. The results showed that area of highest concentration of BNP gene expression were located in the hypothalamus and in the verge area of the thalamus.Sucesscively decreased concentration could also be found in amydaloid nuclei, olfactory bulb, hippocampus and cerebral cortex.

Key words: Brain natriuretic peptide(BNP);In-situ hybridization;Rat brain;mRNA

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引用本文：

刘兢, 马咏, 李菁, 张爱民, 刘宏, 徐洵. 大鼠脑内脑钠素mRNA分布的原位杂交[J]. 生理学报 1994; 46 (2): .

Liu Jing, Ma Yong, Li Jing, Zhang Aimin, Liu Hong, Xu Xun. Detection of messenger RNA coding for BNP precursor in rat brain by in situ hybridization. Acta Physiol Sin 1994; 46 (2): (in Chinese with English abstract).