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神经降压素对醋氨酚引起肝损伤的保护作用及其与谷胱甘肽系统的关系

李继尧, 王玲, 张席锦

北京医科大学生理教研室. 北京(100083)

摘要

神经降压素(NT)在小鼠整体和离体肝细胞均能减轻醋氨酚诱导的转氨酶的漏出, 且在离体肝细胞部分翻转了醋氨酚引起的DNA合成速率的下降。在离体肝细胞醋氨酚使细胞内还原型谷 胱甘肽(GSH), 谷胱甘肽总含量和谷胱甘肽过氧化物酶(GSH-Px)活性均降低, 但氧化型谷胱甘肽(GSSG)含量无明显改变。NT预处理后再给予醋氨酚, 则GSH含量进一步下降, 而GSSG、 谷胱甘肽总含量及GSH-Px活性均增加, 提示NT一方面促进谷胱甘肽的合成, 另一方面通过 提高GSH-Px的活性, 促进GSH转变成GSSG, 从而提高了肝细胞清除自由基的功能。

关键词： 神经降压素; 醋氨酚; 肝细胞保护; 谷胱甘肽

Cytoprotective effect of neurotensin on acetaminophen induced liver injury in relation to glutathione system

Li Jiyao, Wang Ling, Zhang Xijin

Department of Physiology, Beijing Medical University. Beijing

Abstract

Pretreatment with NT significantly reduced the leakages of transaminases induced by acetaminophen in vivo or in cultured hepatocytes and partially reversed the decline of DNA synthesis induced by acetaminophen in cultured hepatocytes.after sadministration of acetaminopohen to cultured hepoatocytes, the contents of reduced glutathione and total glutathione decreased, the activity of glutathione ne peroxydase decreased, but the contents of oxidized glutathione showed no change.Pretreatment with NT before acetaminophen decreased the contents of GSH further but increased the contents of GSSG and tota gultathione and enhanced the activity of GSH_Px.

Key words: Neurotensin;Acetaminophen;Hepatoprotection;Glutathione

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引用本文：

李继尧, 王玲, 张席锦. 神经降压素对醋氨酚引起肝损伤的保护作用及其与谷胱甘肽系统的关系[J]. 生理学报 1994; 46 (2): .

Li Jiyao, Wang Ling, Zhang Xijin. Cytoprotective effect of neurotensin on acetaminophen induced liver injury in relation to glutathione system. Acta Physiol Sin 1994; 46 (2): (in Chinese with English abstract).