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八肽胆囊收缩素拮抗NDAP对大鼠脊髓钙调蛋白的作用

陈素珍, 韩济生

北京医科大学生理教研室. 北京(100083)

摘要

八肽胆囊收缩素拮抗(CCK-8)可通过CCK受体介导增加大鼠脊髓背柱突触小体钙调蛋白(CaM)活性, 并能拮抗NDAP对CaM活性的抑制作用, CCK-8的这一作用可能是其抗阿片作用的机理之一 。

关键词： 胆囊收缩素-8(CCK-8); NDAP; 钙调蛋白; 突触小体

Cholecystokinin octapeptide antagonized the effect of NDAP on CaM in rat dorsal spinal synaptosome

Chen Suzhen, Han Jisheng

Department of Physiology, Beijing Medical University. Beijing

Abstract

The effects and interaction of CCK-8 and k opiate agonist NDAO on CaM activity in rat dorsal spinal cord synaptosomes are observed. The result indicated that 10nmol/L=1umol/L NDAO produced dose-dependent decrease in CaM activity of rat spinal synaptosomes. This suppressive effect was completely blocked by specific k receptor antagonist Nor-BNI at 1 umol/L. In constrast CCK-8 induced a significant increase in CaM activity, and was capable of antagonizing the suppressive effect induced by NDAP. Specific CCK-B receptor antagonist L365,260 could partially block the effects of CCK-8 increased CaM activity at 1 umol/L. The above results suggest that CCK-8 can antagonize the suppressive effect of NDAP on CaM activity in rat spinal synaptosomes via activition of CCK_B receptor, an effect that may constituti one of the mechanisms for the anti-opioid activity of CCK-8.

Key words: CCK-8;NDAP;CaM;Synaptosome

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引用本文：

陈素珍, 韩济生. 八肽胆囊收缩素拮抗NDAP对大鼠脊髓钙调蛋白的作用[J]. 生理学报 1994; 46 (2): .

Chen Suzhen, Han Jisheng. Cholecystokinin octapeptide antagonized the effect of NDAP on CaM in rat dorsal spinal synaptosome. Acta Physiol Sin 1994; 46 (2): (in Chinese with English abstract).