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乙酰胆碱对小鼠胰岛素B细胞电活动作用的分析

陶锋, 苏清芬, 张镜如

上海医科大学生理学教研室. 上海(200032)

摘要

乙酰胆碱(ACh)增强小鼠胰岛素B细胞葡萄糖依赖的电活动是由M受体介导的, 主要为M_(1)亚型, ACh能激活离子通道, 其中有河豚毒敏感的Na通道, 发生Na~(+)内流而使膜 去极化; ACh可能使对异搏定不敏感的Ca通道开放, 发生Ca~(2+)内流而导致锋电位数增多。

关键词： 胰岛B细胞; 乙酰胆碱; 细胞内电位记录; 微电泳

Analysis of the acetylcholine action on the electrical activites of pancreatic islet B-cells in mice

Tao Feng, Su Qingfen, Zhang Jinru

Department of Physiology, Shanghai Medical University. Shanghai

Abstract

The B cell membrane potential was decreased and the spikes were in creased by the ACh microinotophoresis. These effects were dependent on glucose and completely blocked by atropine. However, pirenzepine could attenuate the electrical activity by approximately 70%.The ACh-induced membrane depolarization was Ca~(2+) independent and blocked by TTX.But the effect of ACh on spikes was Ca~(2+)-dependent and not blocked by verapamil.

Key words: Pencreatic Bcells;Acetylcholine;Intracellular potential recording; Microiontophoresis

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引用本文：

陶锋, 苏清芬, 张镜如. 乙酰胆碱对小鼠胰岛素B细胞电活动作用的分析[J]. 生理学报 1994; 46 (2): .

Tao Feng, Su Qingfen, Zhang Jinru. Analysis of the acetylcholine action on the electrical activites of pancreatic islet B-cells in mice. Acta Physiol Sin 1994; 46 (2): (in Chinese with English abstract).