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金属硫蛋白抗内皮素损伤膜蛋白的电子顺磁共振自旋标记研究

王刚, 侯琳, 程时, 唐朝枢, 安蓉姝

北京医科大学生物物理教研室；北京医科大学病理生理教研室；中日友好医院临床医学研究所

摘要

内皮素(ET)(1×10~(-9), 10~(-8), 10~(-7)mol/L)对大鼠红细胞膜膜脂流动性无明显影响, 但在5×10~(-9)及1×10~(-7)mol/L/浓度下均改变膜蛋白构象, 且对膜蛋白构象的影响呈剂量依赖性。1×10~(-5)mol/L金属硫蛋白能抵抗5×10~(-9)mol/L ET对膜蛋白的损伤, 保护生物膜。图2表2参12

关键词： 内皮素; 金属硫蛋白; 红细胞膜; 电子自旋共振

The protective effects of metallothionein on membrane proteins against endothelin: electron paramagnetic resonance spin labelling study

Wang Gang, Hou Lin, Cheng Shi, Tang Chaoshu, An Rongshu

Dept. Biophys., Beijing Med. Univ.；Dept. Pathophysiol., Beijing Med. Univ.；Inst. Clin. Med., Chin-Japanese Friendship Hosp.

Abstract

The lipid fluidity in both of the polar region and the hydrophobic core in the membranes was not altered by endothelin at the concentration of 1×10~(-9), 1×10~(-7) mol/Km but the ratio of S/W of membrane proteins was significantly changed by endothelin at 5×10~(-9) and 1×10~(-7) mol/L.Metallothinojneijn could attenuate this change and protect membrane proteins form endothelin.

Key words: Endothelin;Metallothionein;Erythrocyte membrpe;ESR spin labelling

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引用本文：

王刚, 侯琳, 程时, 唐朝枢, 安蓉姝. 金属硫蛋白抗内皮素损伤膜蛋白的电子顺磁共振自旋标记研究[J]. 生理学报 1994; 46 (1): .

Wang Gang, Hou Lin, Cheng Shi, Tang Chaoshu, An Rongshu. The protective effects of metallothionein on membrane proteins against endothelin: electron paramagnetic resonance spin labelling study. Acta Physiol Sin 1994; 46 (1): (in Chinese with English abstract).