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ATP对单个肺动脉内皮细胞胞浆游离钙浓度的影响

胡清华, 王迪浔, 张宇, 王金唏, 陈荣三

同济医科大学病理生理学教研室. 湖北,武汉(430030)；南京大学配位化学国家重点实验室. 江苏,南京(210009)

摘要

应用Fura-2/AM和双波长显微荧光分光光度计测定原代培养的单个猪肺动脉内皮细胞胞浆游离钙浓度([Ca~(2+)]i)为107±14nmol/L(n=10); 三磷酸腺苷使[Ca~(2+)]i呈 双相增加: 初相峰型, 其后的第二相为平台期。峰相系内钙释放引起, 平台期源于外钙内流图2参7

关键词： 内皮细胞; 胞浆游离钙; 三磷酸腺苷

Effect of ATP on cytosolic free calcium in single porcine pulmonary artery endothelial cell in primary culture

Hu Qinghua, Wang Dixun, Zhang Yu, Wang Jinxi, Chen Rongsan

Department of Pathophysiology, Tongji Medical University. Wuhan,Hubei；China(210009)

Abstract

Using a dual-wavelength excitation fluorescence spectrophotometer, it was found that ATP evoked a rapid rise in [Ca~(2+)]i followed by a slightly maintained increase, Removal of extracellar Ca~(2+)abolished the maintained increase without obvious effect on the initial transient. These results suggest that ATP stimulates both calcium release from intracellular calcium pool(s) and calcium influx across the plasma membrane.

Key words: Cytosolic free;Calcium endothelial;Cell ATP

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引用本文：

胡清华, 王迪浔, 张宇, 王金唏, 陈荣三. ATP对单个肺动脉内皮细胞胞浆游离钙浓度的影响[J]. 生理学报 1994; 46 (4): .

Hu Qinghua, Wang Dixun, Zhang Yu, Wang Jinxi, Chen Rongsan. Effect of ATP on cytosolic free calcium in single porcine pulmonary artery endothelial cell in primary culture. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).