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油菜素甾酮的~(125)I标记及其生物活性

徐如涓, 王玉琴, 吴登如, 何宇炯, 赵毓橘, 方军

中科院上海植物生理研究所, 上海(200032)；上海中医学院. 上海(200032)

摘要

油菜素甾酮的B环-6-酮基经与盐酸羧甲基羟胺反应后得到油菜素甾肟,当浓度为10μmol/L时用水稻叶片倾斜法测得其生物活性的对照增加60%以上;当浓度为2μmol/L时能促使黄瓜下胚轴伸长。用氯胺-T法制备~(125)I-组胺,再与油菜素甾肟结合, 用黄瓜下胚轴 伸长法和水稻叶片倾斜法鉴定活性, Rf0.93处的组分能使水稻幼苗叶片的倾斜活性增加25%, 其放射性比强为73.8GBq/mmol。图5参10

关键词： 油菜素内酯; 油菜素甾酮; 油菜素甾肟; 水稻叶片; 黄瓜下胚轴

Preparation of ~(125)I-brassinosterone and its biological activity

Xu Rujuan, Wang Yuqin, Wu Dengru, He Yujiong, Zhao Yuju, Fang Jun

Shanghai Institute of Plant Physiology, Academia Sinica.Shanghai；China(200032)

Abstract

Brassinosterone, a precursor in the synthesis of brassinolide, exhibitedmore than 70% of the biological activity of brassinolide in rice test.Brassino-oxime, a product of condensation of brassinosterone withcarboxymethoxylamine hydrochloride, showed 60% of that of brassinolide in thesame test system at 10 #mu#mol/L concentration. In cucumber hypocotyl test,the brassion-oxime(2#mu#mol/L)promoted elongation of cucumber hypocotyl by 20%as compared with control. The brassino-oxime was found to be competitive withbrassinolide or brassinosterone. Combination of iodohistamine withbrassion-oxime by Chloramine-T method formed the ~(125)I-labelled compoundwhich was used for cucumber and rice tests. The results showed that the Rf0.93 fraction of chromatography was effective in promoting both elongation andinclination significantly. Its specific radioactivity was 73.8 GBq/mmol.

Key words: Btassinolide;Brassinooterone;Brassino-oalme;Rice lamina;Cucumber hypeotyl

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引用本文：

徐如涓, 王玉琴, 吴登如, 何宇炯, 赵毓橘, 方军. 油菜素甾酮的~(125)I标记及其生物活性[J]. 生理学报 1994; 46 (2): .

Xu Rujuan, Wang Yuqin, Wu Dengru, He Yujiong, Zhao Yuju, Fang Jun. Preparation of ~(125)I-brassinosterone and its biological activity. Acta Physiol Sin 1994; 46 (2): (in Chinese with English abstract).