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川芎嗪增加大鼠远端结肠阴离子分泌的基侧膜机制

邢莹, 何琼, 朱进霞, 陈小章

郑州大学医学院生理学教研室.河南,郑州 450052；香港中文大学医学院上皮细胞生物学研究中心.香港

摘要

研究用短路电流技术来观察在川芎嗪作用下,电解质在大鼠远端结肠上皮细胞的转运及其细胞机制。在新鲜分离的结肠上皮的基侧膜加入川芎嗪,能产生较大的短路电流。用粘膜下神经元阻断剂--河豚毒素预作用于结肠上皮,不影响随后的川芎嗪所产生的短路电流,前列腺素合成抑制剂indomethacin预作用可使随后的川芎嗪产生的短路电流减少55.2%。在结肠上皮的顶膜加入Cl~(-)通道阻断剂DPC和glibenclamide,能完全阻断川芎嗪产生的短路电流。Bumetanide,基侧膜钠、钾、氯共转运体阻断剂能抑制川芎嗪引起的短路电流的85.2%,而结肠上皮细胞基侧膜的非选择性钾通道阻断剂Ba~(2+)能阻断90%以上的短路电流,说明基侧膜的钠、钾、氯共转运体和钾通道在川芎嗪引起的短路电流中起着重要作用。上述结果表明,川芎嗪刺激大鼠远端结肠上皮细胞分泌Cl~(-)是通过上皮细胞顶膜Cl~(-)通道和基侧膜的钠、钾、氯共转体和K~(+)通道介导的。

关键词： 川芎嗪; 结肠上皮; C1-分泌; Na+-K+-2Cl-共转运体; K+通道

Basolateral membrane mechanisms involved in ligustrazine-stimulated anion secretion in rat distal colon

Xing Ying, He Qiong, Zhu Jinxia, Chan Hsiaochang

Department of Physiology,Medical School,Zhengzhou University.Zhengzhou 450052,Henan；China

Abstract

The present study investigated the cellular mechanism underlying the effect of ligustrazine on the ion transport in rat distal colon using the short-circuit current (I_(SC)) technique. In freshly isolated colonic strips, basolateral addition of ligustrazine stimulated a rise in I_(SC), which was resistant to basolateral application of neuronal sodium channel blocker tetrodotoxin (TTX), but inhibited by 55.2% by basolateral pretreatment with prostaglandin inhibitor indomethacin. The ligustrazine-induced I_(SC) increase was inhibited by apical application of Cl~(-) channel blockers diphenylamine-2, 2^-dicarboxylic acid (DPC) and glibenclamide. Basolaterally administered bumetanide, an inhibitor of Na~(+)-K~(+)-2Cl~(-) cotransporter, inhibited ligustrazine-evoked current increases by 85.2% and basolateral exposure to Ba~(2+), a non-specific potassium channels blocker, and blocked the current by more than 90%, indicating that basolateral Na~(+)-K~(+)-2Cl~(-) cotransporter and K~(+) channels played an important role in the effect of ligustrazine. The results suggested that ligustrazine could stimulate rat distal colon Cl~(-) secretion that is mediated by basolateral Na~(+)-K~(+)-2Cl~(-) cotransporter and K~(+) channel.

Key words: Ligustrazine;Colonic mucosa;C1 secretion;Na+-K + -2C1 cotransporter;K+ channels

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引用本文：

邢莹, 何琼, 朱进霞, 陈小章. 川芎嗪增加大鼠远端结肠阴离子分泌的基侧膜机制[J]. 生理学报 2003; 55 (6): .

Xing Ying, He Qiong, Zhu Jinxia, Chan Hsiaochang. Basolateral membrane mechanisms involved in ligustrazine-stimulated anion secretion in rat distal colon. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).