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培养牛心内膜内皮细胞的腺苷三磷酸双磷酸酶的特性研究

易富贤, 孙平, 黄韶玲, 刘文兰, 郭兆贵

湖南医科大学分子药理研究室.湖南,长沙 410078

摘要

以无机磷释放法检测培养牛心内膜内皮细胞(BEEC)apyrase的活性。结果表明,牛心内膜内皮细胞存在叠氮钠敏感的、依赖于二价阳离子和pH值的腺苷三磷酸双磷酸酶活性。

关键词： 腺苷三磷酸双磷酸酶; 腺苷二磷酸; 心内膜; 叠氮钠

Characteristics of apyrase (EC 3.6.1.5) on cultured bovine endocardial endothelial cells

Yi Fuxian, Sun Ping, Huang Shaoling, Liu Wenlan, Guo Zhaogui

Laboratory of Molecular Pharmacology,Hunan Medical University.Changsha 410078,Hunan

Abstract

Apyrase activity was assayed by inorganic phosphate release, which could be inhibited concentration-dependently by NaN_(3), an apyrase inhibitor. NaF (20 mmol/L), another inhibitor of apyrase, also markedly inhibited the activity. EDTA or EGTA (1 mmol/L) could also inhibit the activity completely. However, the inhibitor for Na~(+)/K~(+)-ATPase, ouabain (3 mmol/L) did not affect the enzyme activity.

Key words: Apyrase;Adenosine diphosphate;Endocardium;Sodium azide

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引用本文：

易富贤, 孙平, 黄韶玲, 刘文兰, 郭兆贵. 培养牛心内膜内皮细胞的腺苷三磷酸双磷酸酶的特性研究[J]. 生理学报 1999; 51 (4): .

Yi Fuxian, Sun Ping, Huang Shaoling, Liu Wenlan, Guo Zhaogui. Characteristics of apyrase (EC 3.6.1.5) on cultured bovine endocardial endothelial cells. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).