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在条锈菌侵染早期小麦初生叶内多聚核糖体水平与蛋白质合成能力的变化

张慧, 商鸿生, 李振岐

西北农业大学植物保护系. 陕西, 杨陵(712100)

摘要

小麦初生叶接种条锈菌毒性生理小种(CY29)及其弱毒突变菌系(CY29-mut 3)后, 呈不亲和 反应的寄主叶片可溶性蛋白质合成能力在接种后24h显著高于未接种对照, 但其后逐渐降低, 直至接近对照; 而呈亲和性反应的寄主叶片可溶性蛋白质合成在侵染早期与对照相近, 但 与膜结合蛋白在96h时大大高于对照。对接种叶中核糖体的密度梯度分析证实: 呈不亲和反 应寄主叶片游离多聚核糖体及亲和反应的寄主内与膜结合多聚核糖体均有特异性增加。表明寄主的抗病和感病反应均与蛋白质合成能力的变化有关。图 2表3参14

关键词： 小麦; 小麦条锈病; 蛋白质体内合成; 多聚核糖体; 抗条锈性

Changes in polyribosome level and protein synthesis of wheat leaves in the early stage of stripe-rust infection

Zhang Hui, Shang Hongsheng, Li Zhenqi

Department of Plant Protection, Northwestern Agricultural University. Yangling, Shaanxi

Abstract

After wheat(Triticum aestivum L.CV Lovrin 13)primary leaves inoculated with virulent race(CY29)of stripe-rust fungus (Puccinia striiformis West) and its avirulent mutant (CY29-mut3), the rate of soluble protein synthesis in vivo in incompatible host leaves was higher than uninoculated leaves 24 h after inoculation, and then declined. In compatible host leaves, however, though soluble protein synthesis was the same as uninoculated leaves during the early stage of infection, a sharp increase in the rate of synthesis of membrane bound protein occurred 96 h after inoculation. Meanwhile, analysis of ribosome in wheat primary leaves by sucrose density gradient centrifugation confirmed that there was predominant increase of free polyribosome level in incompatible host and of membrane bound polyribosome level in compatible host. It is conclued that reactions of both the resistant and susceptible host plants involve alterations in protein synthesis ability.

Key words: Wheat;Wheat stripe rust;In vivo protein synthesis;Ployribosome;Rust resistance

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引用本文：

张慧, 商鸿生, 李振岐. 在条锈菌侵染早期小麦初生叶内多聚核糖体水平与蛋白质合成能力的变化[J]. 生理学报 1994; 46 (4): .

Zhang Hui, Shang Hongsheng, Li Zhenqi. Changes in polyribosome level and protein synthesis of wheat leaves in the early stage of stripe-rust infection. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).