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云南松花粉中钙调素结合蛋白的分离和鉴定

龚明, 杨中汉, 曹宗巽

北京大学生物系. 北京(100871)

摘要

通过CaM-Sepharose 4B亲和层析方法从云南松花粉中提取出10种CaM结合蛋白。它们均能抑制CaM对PDE的激活, 但这种抑制可被随后加入的过量的CaM所消除。酶活测定表明CaM结合蛋白中有Ca~(2+)-CaM依赖的ATPase活力, 但无植酸酶、过氧化物酶、酸性磷酸酶和磷酶D活性。图4表1参16

关键词： 钙调素结合蛋白; ATP酶; 花粉; 云南松

Isolation and identification of calmodulin-binding proteins from Pinus yunnanensis pollen

Gong Ming, Yang Zhonghan, Cao Zongxun

Department of Biology, Peking University. Beijing

Abstract

Ten CaM-binding proteins were isolated from Pinus yunnanensis pollen by CaM-Sepharose 4B affinity chromatography. These proteins could inhibit CaM-activated PDE activity, which could be eliminated by adding-an excess of exogenous CaM. The assay of enzyme activity indicated that there were Ca~(2+)-and CaM-dependent ATPase activities, but no peroxidase, phytase, acid phosphatase and phospholipase D activities in CaM-binding proteins of pine pollen.

Key words: CaM-binding proteins;ATPase;Pollen;Pinus yunnanensis

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引用本文：

龚明, 杨中汉, 曹宗巽. 云南松花粉中钙调素结合蛋白的分离和鉴定[J]. 生理学报 1994; 46 (4): .

Gong Ming, Yang Zhonghan, Cao Zongxun. Isolation and identification of calmodulin-binding proteins from Pinus yunnanensis pollen. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).