过刊浏览

Thidiazuron对苜蓿愈伤组织的乙烯生成及其体细胞胚胎发生的影响

黄学林, 李筱菊, 傅家瑞, 劳彩玲

中山大学生命科学院生物学系. 广东,广州(510275)；中山大学测试中心. 广东,广州(510275)

摘要

苜蓿叶柄在B_(5)H培养基上诱导并继代所形成的是无色、松软的胚性愈伤组织, 内含许多有分生能力的细胞团。当B_(5)H中的KT用TDZ替换时, 愈伤组织变绿变硬, 出现管状 分子和芽原基的分化, 其体胚发生能力丧失, 而且其乙烯生成和PAL活性升高。乙烯形成酶 的抑制剂CoCl_(2)可以抑制TDZ的上述作用, 部分恢复愈伤组织的体胚发生能力。TDZ对愈伤组织的这些作用可能与其促进乙烯生成有关。图版1表4参25

关键词： 苜蓿愈伤组织; 体细胞胚胎发生; 乙烯; 苯丙氨酸解氨酶; CoCl_(2); Thidiazuron

Effect of thidiazuron on ethylene production and somatic embryogenesis in callus of alfalfa (Medicaga sativa L.)

Huang Xuelin, Li Xiaoju, Fu Jiarui, Lao Cailin

Department of Biology, Zhongshan University. Guangzhou,Guangdong；China(510275)

Abstract

Callus initiation from the petiole, induction and development of the somatic embryos occurred in medium B_(5)H, B_(5)g and SH (hormone-free) with proline 30 mmol/L and (NH_(4))_(2)SO_(4) 10 mmol/L respectively. When kinetin in B_(5)H was replaced by TDZ, the colorless, friable and embryogenic callus in B_(5)H become green, campact and nonembryogenic. TDZ increased ethylene production and PAL activity of the callus 3 to 4-fold and 2 fold, respectively, after 3 to 4 week culture. Histological observation revealed that some tracheary elements and their strands were differentiated from the callus with TDZ, but meristem-like structure from control callus. CoCl_(2), an inhibitor of ethylene forming-enzyme, was found to countereact to the effects of TDZ and the effects of CoCl_(2) depend on its concentration. The callus can be changed back colorless, friable one, and its capability of undergoing somatic embryogenesis recovered to some extent, but its ethylene production was reduced, or even stopped, by treatment with CoCl_(2).

Key words: Callus of Alfalfa;Somatic embryogenesis;Ethylene;Phenylalanine ammonia-lyase (PAL);CoCl_(2);Thidiazuron

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

黄学林, 李筱菊, 傅家瑞, 劳彩玲. Thidiazuron对苜蓿愈伤组织的乙烯生成及其体细胞胚胎发生的影响[J]. 生理学报 1994; 46 (4): .

Huang Xuelin, Li Xiaoju, Fu Jiarui, Lao Cailin. Effect of thidiazuron on ethylene production and somatic embryogenesis in callus of alfalfa (Medicaga sativa L.). Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).