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外源GUS基因在PEG介导的花椰菜原生质体中的瞬间表达

杨仲南, 许智宏, 白永延, 卫志明

中科院上海植物生理研究所植物分子遗传国家重点实验室. 上海(200032)

摘要

20%PEG将质粒pBI221导入原生质体后GUS表达比13%PEG导入的高, 但易造成原生质体损伤。 热激处理增强表达, 但在随后的培养过程中易造成原生质体降解。原生质体状况对表达有重要影响, 5d龄下胚轴原生质体比8d龄的表达强; 不同质粒及启动子表达强度不同。质粒pKIWI101比pBI221表达强3倍左右。表4参12

关键词： 花椰菜; 下胚轴; 原生质体; PEG; 瞬间表达

The transient gene expression of exogenous GUS genes in cauliflower hypocotyl protoplasts

Yang Zhongnan, Xu Zhihong, Bai Yongyan, Wei Zhiming

National Key Lab of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai

Abstract

The transient expression of GUS gene mediated by 20% PEG was higher than that mediated by 13% PEG. However, 13% PEG was more benificial than 20% PEG for high protoplast survival; Heat shock improved the expression, but it was harmful to protoplasts; The state of protoplasts affected the expression greatly. The Gus gene expression in protoplasts isolated from 5-day-old hypocotyls was much stronger than that from 8-day-old hypocotyls; Plasmid pKI-WI101 gave a much stronger transient expression than plasmid pBI221.

Key words: Cauliflower;Hypocotyl;Protoplast;PEG;Transient expression

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引用本文：

杨仲南, 许智宏, 白永延, 卫志明. 外源GUS基因在PEG介导的花椰菜原生质体中的瞬间表达[J]. 生理学报 1994; 46 (3): .

Yang Zhongnan, Xu Zhihong, Bai Yongyan, Wei Zhiming. The transient gene expression of exogenous GUS genes in cauliflower hypocotyl protoplasts. Acta Physiol Sin 1994; 46 (3): (in Chinese with English abstract).