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光质对黄瓜及番茄愈伤组织培养中分化和有关酶的影响

王维荣, 王咏冬, 欧阳光察, 薛应龙

复旦大学生物化学系

摘要

黄瓜及番茄愈伤组织培养过程中的分化和生理生化过程明显地受到光质处理 的影响, 它们的过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)的活性变化与组织及 器官的分化都有一定的相关性。黄瓜愈伤组织培养中POD及PAL的第3 个活性峰的 出现与芽的分化一致, 红光与绿光抑制黄瓜愈伤组织培养中芽的分化, 不出现 第3 个活性峰。两种材料培养后培养物的生物重量在不同光质下也有明显差异, 但含水率之间无差异。图4表2参20

关键词： 黄瓜; 番茄; 光质; 组织培养; 分化; 酶

Effects of light quality on differentiation and its related enzymes in callus culture of cucumber and tomato

Wang Weirong, Wang Yongdong, Ouyang Guangcha, Xue Yinglong

Dept. Biochem., Fudan Univ.

Abstract

Light quality has a marked effect on differentiation and physiological and biochemical process of cultured cucumber and tomato calluses. The changes of activities of these enzymes were positively correlated to the differentiation of tissue and organ in culture process. The third ones under white, blue, and yellow lights and in the dark were related to the differentiation of buds in cucumber callus cultures. Red and green lights inhibited bud formation, and no third peak was seen. Light quality also affected the biomass of the cultured calluses of the two plants during culture.

Key words: Light quality;Callus culture;Differentiation;Perioxidase;Phenylalanine ammonia lyase;Cucumis sutivis

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引用本文：

王维荣, 王咏冬, 欧阳光察, 薛应龙. 光质对黄瓜及番茄愈伤组织培养中分化和有关酶的影响[J]. 生理学报 1991; 43 (2): .

Wang Weirong, Wang Yongdong, Ouyang Guangcha, Xue Yinglong. Effects of light quality on differentiation and its related enzymes in callus culture of cucumber and tomato. Acta Physiol Sin 1991; 43 (2): (in Chinese with English abstract).