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寡霉素在叶绿体中的作用部位

卫瑾

中国科学院上海植物生理研究所

摘要

寡霉素对在光下以胰蛋白酶激活的叶绿体膜上Mg~(2+)-ATPase和脱离了 膜的Ca~(2+)-TPase都有抑制作用, 但对经NEM修饰的叶绿体膜上Mg~(2+)- ATPase活力没有影响, 促进 质子流出的效应也消失, 在暗中经胰蛋白酶活化的 Ca ~(2+)-ATPase对寡霉素不敏感。推断寡霉素抑制光激活的ATPase 和促进 质子流出的效应是光引起膜能化导致CF_(1)变构, γ亚基暴露, 使寡霉素能 与之结合的结果, 寡霉素在叶绿体上的作用部位是在CF_(1)中, 与线粒体在 F_(0) 上有所不同。图5表3参14

关键词： 叶绿体; 寡霉素

Study on the action site of oligomycin in chloroplast

Wei Jin

Shanghai Inst. Plant Physiol., Acad. Sin.

Abstract

The inhibitory effect of oligomycin on the activity of membrane Mg super(2+)-ATpase from CF sub(1) on membrane and Ca super(2+)-ATPase of soluble CF sub(1). Illumination induced some conformational changes of the enzyme, which caused the exposure of GAMMA-subunit and easy digestion by trypsin. Both ATPases with exposed GAMMA-subunit were highly sensitive to oligomycin, the action site of which was shown to be on the GAMMA-subunit of CF sub(1). The Mg super(2+)-ATPase activated by TPCK-trypsin of chloroplast treated with NEM, a GAMMA-subunit modifying agent was not affected by oligomycin.

Key words: Oligomycin;NEM

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引用本文：

卫瑾. 寡霉素在叶绿体中的作用部位[J]. 生理学报 1991; 43 (2): .

Wei Jin. Study on the action site of oligomycin in chloroplast. Acta Physiol Sin 1991; 43 (2): (in Chinese with English abstract).