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肠黏膜通透性的评估方法

严婧婷^1,2, 孙齐¹, 张晓丽¹, 刘晶华³, 冯小燕¹, 朱进霞^1,*

¹首都医科大学基础医学院生理与病理生理学系，北京 100069；²山东第一医科大学第一附属医院(山东省千佛山医院)麻醉科，山东省麻醉与呼吸危重症研究所，济南 250000；³首都医科大学第六临床医学院，北京 100029

摘要

肠黏膜屏障(intestinal mucosal barrier, IMB)包括机械屏障、化学屏障、生物屏障和免疫屏障，在维持肠上皮的完整性，防御细菌、内毒素及抗原的入侵等方面发挥着重要作用。IMB受损表现为肠黏膜通透性(intestinal mucosal permeability, IMP)增加以及跨膜电阻(transmembrane resistance, TR)降低，与消化、泌尿、循环、神经、代谢等机体多系统功能紊乱密切相关。利用短路电流(short circuit current, SCC)记录系统或跨上皮细胞电阻仪(transepithelial electrical resistance meter)检测肠黏膜或肠上皮电阻，以及生化检测外源性糖分子探针(口服)或特定内源性蛋白分子跨肠黏膜或肠上皮通透量，是评价肠黏膜或肠上皮通透性的常用方法。本文简介IMB的组成及其功能，重点讨论IMP的评估方法。

关键词： 肠黏膜; 肠黏膜通透性; 肠上皮电阻; 糖分子探针

Methods for the evaluation of intestinal mucosal permeability 

YAN Jing-Ting^1,2, SUN Qi¹, ZHANG Xiao-Li¹, LIU Jing-Hua³, FENG Xiao-Yan¹, ZHU Jin-Xia^1,*

¹Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China；²Department of Anesthesiology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Institute of Anesthesia and Respiratory Critical Medicine, Jinan 250000, China；³Sixth Clinical Medical College, Capital Medical University, Beijing 100029, China

Abstract

The intestinal mucosal barrier (IMB), which consists of mechanical barrier, chemical barrier, biological barrier and immune barrier, plays an important role in the maintenance of intestinal epithelium integrity and defense against invasion of bacteria, endotoxins and foreign antigens. Impaired IMB, characterized by increased intestinal mucosal permeability (IMP) and decreased transmembrane resistance (TR), has been implicated in the pathogenesis of various digestive, urinary, circulatory, neurological and metabolic dysfunctions. Electrophysiological recording of TR in the ex vivo intestinal tissues or cultured epithelial cell monolayers, or biochemical quantification of transepithelial movement of orally-administered molecular probes or specific endogenous protein molecules has frequently been used in the evaluation of IMB. In this paper, the composition and function of IMB will be summarized, with emphasis on the evaluation methods of IMP. 

Key words: intestinal mucosa; intestinal mucosal permeability; intestinal epithelial resistance; sugar molecular probe

收稿日期：　　录用日期：

通讯作者：朱进霞　　E-mail: 1959zhujinxia@163.com

DOI: 10.13294/j.aps.2022.0014

引用本文：

严婧婷, 孙齐, 张晓丽, 刘晶华, 冯小燕, 朱进霞. 肠黏膜通透性的评估方法[J]. 生理学报 2022; 74 (4): 596-608.

YAN Jing-Ting, SUN Qi, ZHANG Xiao-Li, LIU Jing-Hua, FENG Xiao-Yan, ZHU Jin-Xia. Methods for the evaluation of intestinal mucosal permeability . Acta Physiol Sin 2022; 74 (4): 596-608 (in Chinese with English abstract).