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大鼠触液核MrgA的分布及其在神经病理性疼痛状态下的表达增加

陈玉凤^1,2, 田恩琪^1,2, 王国平¹, 周芳², 张励才^2,*

¹长治市人民医院麻醉科，长治 046000；²徐州医科大学江苏省麻醉学重点实验室，徐州 221000

摘要

本文旨在观察MrgA (Mas-related G protein-coupled receptor A)在正常大鼠触液核的分布及其在神经病理性疼痛条件下的表达变化，为触液核通过MrgA参与神经病理性疼痛的信息传递或调节提供形态学依据。按照文献建立坐骨神经慢性结扎损伤(chronic constriction injury of sciatic nerve, CCI)大鼠模型，用Von Frey电子测痛仪和热痛敏刺激仪监测大鼠痛行为，用霍乱毒素B亚单位结合辣根过氧化物酶(CB-HRP)追踪和免疫荧光标记相结合的方法来检测并比较MrgA在正常和CCI大鼠触液核的表达及变化。结果显示，CCI大鼠第5、7、10、14天的机械缩足反射阈值和热缩足潜伏期显著降低，MrgA在正常大鼠触液核有分布，CCI大鼠神经病理性疼痛达到峰值时触液核MrgA表达水平显著高于正常对照组。以上结果提示，触液核可能通过MrgA参与了神经病理性疼痛的信息传递或调节。

关键词： 触液核; MrgA; 神经病理性疼痛

The distribution of Mas-related G protein-coupled receptor A in cerebrospinal fluid-contacting nucleus of normal rats and its up-regulation in neuropathic pain

CHEN Yu-Feng^1,2, TIAN En-Qi^1,2, WANG Guo-Ping¹, ZHOU Fang², ZHANG Li-Cai^2,*

¹Department of Anesthesiology, Changzhi People’s Hospital, Changzhi 046000, China；²Jiangsu Provincial Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou 221000, China

Abstract

This study was aimed to observe the distribution of Mas-related G protein-coupled receptor A (MrgA) in cerebrospinal fluid (CSF)-contacting nucleus of normal rats and its expression in neuropathic pain, and to provide morphological evidence for CSF- contacting nucleus to participate in neuropathic pain. The model of neuropathic pain with chronic constriction injury (CCI) of the sciatic nerve was made in Sprague-Dawley rats. The thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were measured. The expressions of MrgA in the CSF-contacting nucleus were examined by double labeling with immunofluorescent staining. The results showed that on the 5th, 7th, 10th and 14th days, the values of MWT and TWL in CCI group were all lower than those in sham group (P < 0.05). MrgA was found to be distributed in CSF-contacting nucleus of normal rats; and the expression was markedly up-regulated in rats at the peak of neuropathic pain. Our data suggest that CSF-contacting nucleus may participate in neuropathic pain through the MrgA-mediated signaling pathway.

Key words: CSF-contacting nucleus; MrgA; neuropathic pain

收稿日期：　　录用日期：

通讯作者：张励才　　E-mail: licaizhang001@163.com

DOI: 10.13294/j.aps.2022.0044

引用本文：

陈玉凤, 田恩琪, 王国平, 周芳, 张励才. 大鼠触液核MrgA的分布及其在神经病理性疼痛状态下的表达增加[J]. 生理学报 2022; 74 (3): 353-358.

CHEN Yu-Feng, TIAN En-Qi, WANG Guo-Ping, ZHOU Fang, ZHANG Li-Cai. The distribution of Mas-related G protein-coupled receptor A in cerebrospinal fluid-contacting nucleus of normal rats and its up-regulation in neuropathic pain. Acta Physiol Sin 2022; 74 (3): 353-358 (in Chinese with English abstract).