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XBP1在小鼠早期妊娠子宫的表达和调节

郭思维¹, 于雯¹, 王煦¹, 刘健慧¹, 张新月¹, 何成强¹, 丁乃峥^1,*

¹山东师范大学生命科学学院，山东省动物抗性实验室，济南 250014

摘要

转录因子X盒结合蛋白1 (X-box binding protein-1, XBP1)在未折叠蛋白反应中起关键作用。本研究旨在分析XBP1在小鼠早期妊娠过程中的表达和调节，探讨其与胚胎着床的相关性。分别建立小鼠早期妊娠、人工诱导蜕膜化、发情周期和激素调节模型，通过免疫组织化学染色和实时定量RT-PCR技术检测XBP1在这些动物模型中的表达模式。结果显示，XBP1在小鼠早期妊娠子宫中呈现动态的变化，第1~4天主要表达在腔上皮和腺上皮，第5~8天主要表达在蜕膜区；在人工诱导蜕膜化的子宫中XBP1主要存在于蜕膜区；在小鼠发情周期模型中，Xbp1、Xbp1u、Xbp1s mRNA在发情前期的表达量最高；在卵巢切除的子宫中，雌激素处理后腔上皮和腺上皮细胞XBP1的表达上调。以上结果表明，XBP1参与小鼠胚胎着床和蜕膜化，其在早期妊娠子宫腔上皮和腺上皮中的表达受到雌激素调控。

关键词： 小鼠; X盒结合蛋白1; 早期妊娠; 子宫; 胚胎着床

分类号：Q25

Expression and regulation of XBP1 in mouse uterus during early pregnancy

GUO Si-Wei¹, YU Wen¹, WANG Xu¹, LIU Jian-Hui¹, ZHANG Xin-Yue¹, HE Cheng-Qiang¹, DING Nai-Zheng^1,*

¹Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Sciences, Shandong Normal University, Jinan 250014, China

Abstract

The transcription factor X-box binding protein-1 (XBP1) plays a key role in unfolded protein reaction. This study was aimed to investigate the expression pattern and regulation of XBP1 in the mouse uterus during early pregnancy. The methods of immunohistochemistry (IHC) and real time quantitative RT-PCR were used to test XBP1 expression in early pregnancy, artificial decidualization, oestrous cycle and hormone-regulated mouse models. The results showed that XBP1 was spatiotemporally expressed in mouse uterus during early pregnancy. The XBP1 protein was mainly detected in the luminal and glandular epithelia on days 1–4 of pregnancy, and was strongly detected in the decidual area on days 5–8 of pregnancy. Similarly, XBP1 expression was also mainly expressed in decidual cells following artificial decidualization. During the oestrous cycle, Xbp1, Xbp1u, and Xbp1s mRNA was predominantly present in proestrus. In the ovariectomized uterus, the expression of XBP1 in luminal and glandular epithelia was up-regulated after estrogen treatment. These results suggest that XBP1 is associated with embryo implantation and decidualization during early pregnancy in mice, and the expression of XBP1 in luminal and glandular epithelia may be regulated by estrogen.

Key words: Mouse; X-box binding protein-1; early pregnancy; uterus; embryo implantation

收稿日期：2020-04-29　　录用日期：2020-10-20

通讯作者：丁乃峥　　E-mail: nzding@sdnu.edu.cn

DOI: 10.13294/j.aps.2020.0091

引用本文：

郭思维, 于雯, 王煦, 刘健慧, 张新月, 何成强, 丁乃峥. XBP1在小鼠早期妊娠子宫的表达和调节[J]. 生理学报 2021; 73 (2): 208-216.

GUO Si-Wei, YU Wen, WANG Xu, LIU Jian-Hui, ZHANG Xin-Yue, HE Cheng-Qiang, DING Nai-Zheng. Expression and regulation of XBP1 in mouse uterus during early pregnancy. Acta Physiol Sin 2021; 73 (2): 208-216 (in Chinese with English abstract).