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黄芪甲苷减轻大鼠脑缺血再灌损伤并抑制NF-κB磷酸化及NLRP3炎症小体活化

唐标, 唐文静, 唐映红, 邓常清^*

湖南中医药大学医学院，长沙 410028

摘要

本文旨在探讨黄芪甲苷(astragaloside IV, AST-IV)在大鼠脑缺血再灌注损伤中的保护作用及其抗炎机制。采用改良线栓法制备大鼠大脑中动脉栓塞脑缺血再灌注模型，以神经功能缺失评分和脑梗死体积综合评价AST-IV抗脑缺血再灌注损伤的作用，Western blot检测脑组织中NLRP3、ASC、pro-Caspase-1、Caspase-1、pro-IL-1β、IL-1β、pro-IL-18、IL-18、磷酸化NF-κB和总NF-κB蛋白表达水平。结果显示，与模型组比较，AST-IV能降低大鼠神经功能缺失评分，减少脑梗死体积，降低脑组织中NLRP3、Caspase-1、pro-IL-1β、IL-1β、pro-IL-18和IL-18蛋白水平，并抑制磷酸化NF-κB蛋白表达。以上结果提示，AST-IV具有抗脑缺血再灌注损伤作用，其机制可能与抑制NF-κB蛋白磷酸化以及抑制NLRP3炎症小体活化有关。

关键词： 黄芪甲苷; 脑缺血再灌注; NF-κB; NLRP3炎症小体; 炎症

分类号：R332;R363.2;R329.2

Astragaloside IV attenuates cerebral ischemia and reperfusion injury and reduces activation of NLRP3 inflammasome and NF-κB phosphorylation in rats following a transient middle cerebral artery occlusion

TANG Biao, TANG Wen-Jing, TANG Ying-Hong, DENG Chang-Qing^*

Medical School, Hunan University of Chinese Medicine, Changsha 410028, China

Abstract

The present study was aimed to investigate the protective effect and anti-inflammation mechanism of astragaloside IV (AST-IV) on cerebral ischemia and reperfusion injury. Following the establishment of cerebral ischemia and reperfusion model in rats by modified suture method, neurological deficit scores and cerebral infarct volume were used to evaluate the pharmacological effect of AST-IV against cerebral ischemia-reperfusion injury. Western blot was used to detect the expression levels of NLRP3, pro-Caspase-1, Caspase-1, pro-IL-1β, IL-1β, pro-IL-18, IL-18, phosphorylated and total nuclear factor kappa B (NF-κB)/p65 protein in the brain tissue. The results showed that compared with model group, the intervention of AST-IV decreased the neurological deficit scores, reduced the cerebral infarct volume, decreased the levels of NLRP3, Caspase-1, pro-IL-1β, IL-1β, pro-IL-18 and IL-18, and inhibited the expression of phosphorylated NF-κB in brain tissue. The results suggest that AST-IV has a protective effect against cerebral ischemia and reperfusion injury, and its mechanism is related to inhibiting the phosphorylation of NF-κB and NLRP3 inflammasome activation. 

Key words: astragaloside IV; cerebral ischemia and reperfusion; NF-κB; NLRP3 inflammasome; inflammation

收稿日期：2018-08-06　　录用日期：2018-11-13

通讯作者：邓常清　　E-mail: dchangq@sohu.com

DOI: 10.13294/j.aps.2019.0007

引用本文：

唐标, 唐文静, 唐映红, 邓常清. 黄芪甲苷减轻大鼠脑缺血再灌损伤并抑制NF-κB磷酸化及NLRP3炎症小体活化[J]. 生理学报 2019; 71 (3): 424-430.

TANG Biao, TANG Wen-Jing, TANG Ying-Hong, DENG Chang-Qing. Astragaloside IV attenuates cerebral ischemia and reperfusion injury and reduces activation of NLRP3 inflammasome and NF-κB phosphorylation in rats following a transient middle cerebral artery occlusion. Acta Physiol Sin 2019; 71 (3): 424-430 (in Chinese with English abstract).