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极低频电磁辐射增强秀丽线虫能量代谢并诱导氧化应激反应

孙永彦^1,2,3,4, 王亚红^1,2,3, 李志辉^1,2,3, 石振华^2,5, 廖艳艳^1,2,3, 唐超^1,3, 蔡澎^1,3,6,*

¹中国科学院城市环境研究所，厦门 361021；²中国科学院大学，北京 100049；³厦门市物理环境重点实验室，厦门 361021；⁴天津理工大学环境科学与安全工程学院，天津 300384；⁵福建农林大学资源与环境学院，福州 350002；⁶中国科学院上海生命科学研究院营养与健康研究所，上海 200031

摘要

本文旨在研究极低频电磁场(extremely low frequency electromagnetic field, ELF-EMF)暴露对秀丽隐杆线虫(以下简称线虫)能量代谢和氧化应激反应的影响。50 Hz、3 mT ELF-EMF暴露，20 °C条件下分别培养线虫56 h (成虫初期)、72 h (产卵期)和90 h (产卵高峰期)，检测三个时期的成虫体内ATP水平、ATP合成酶活性、活性氧(reactive oxygen species, ROS)含量以及线虫总抗氧化能力(total antioxidant capacity, TAC)的变化情况，并评估ELF-EMF暴露对线虫氧化应激(ROS-TAC分数)的影响。结果表明，经ELF-EMF暴露的线虫与对照组比较，体内ATP含量上升，ATP合成酶活性上调；在成虫初期，与对照组比较，线虫ROS水平经ELF-EMF暴露后显著增加，同时TAC增强，主成分分析法比较二者变化程度提示ROS-TAC分数显著降低，表明ELF-EMF引起成虫初期氧化应激反应。产卵期与产卵高峰期成虫氧化应激反应较对照组无显著差异。以上结果提示，50 Hz极低频电磁辐射可致成虫能量代谢增强并引起成虫初期氧化应激反应。

关键词： 能量代谢; 氧化应激; 电磁辐射; 秀丽线虫

分类号：R730.2; Q493.8

Extremely low frequency electromagnetic radiation enhanced energy metabolism and induced oxidative stress in Caenorhabditis elegans

SUN Yong-Yan^1,2,3,4, WANG Ya-Hong^1,2,3, LI Zhi-Hui^1,2,3, SHI Zhen-Hua^2,5, LIAO Yan-Yan^1,2,3, TANG Chao^1,3, CAI Peng^1,3,6,*

¹Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China；²University of Chinese Academy of Sciences, Beijing 100049, China；³Xiamen Key Laboratory of Physical Environment, Xiamen 361021, China；⁴College of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China；⁵College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China；⁶Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

Abstract

The aim of this study was to determine the effects of extremely low frequency electromagnetic field (ELF-EMF) on energy metabolism and oxidative stress in Caenorhabditis elegans (C. elegans). Worms in three adult stages (young adult stage, egg-laying stage and peak egg-laying stage) were investigated under 50 Hz, 3 mT ELF-EMF exposure. ATP levels, ATP synthase activity in vivo, reactive oxygen species (ROS) content, and changes of total antioxidant capacity (TAC) were detected, and worms’ oxidative stress responses were also evaluated under ELF-EMF exposure. The results showed that ATP levels were significantly increased under this ELF-EMF exposure, and mitochondrial ATP synthase activity was upregulated simultaneously. In young adult stage, worms’ ROS level was significantly elevated, together with upregulated TAC but with a decreased ROS-TAC score indicated by principal component analysis. ROS level and TAC of worms had no significant changes in egg-laying and peak egg-laying stages. Based on these results, we concluded that ELF-EMF can enhance worm energy metabolism and elicit oxidative stress, mainly manifesting as ATP and ROS level elevation together with ATP synthase upregulation and ROS-TAC score decrease in young adult C. elegans. 

Key words: Energy metabolism; oxidative stress; electromagnetic field; C. elegans

收稿日期：2018-08-23　　录用日期：2019-01-19

通讯作者：蔡澎　　E-mail: pcai@iue.ac.cn; pcai@sibs.ac.cn

DOI: 10.13294/j.aps.2019.0030

引用本文：

孙永彦, 王亚红, 李志辉, 石振华, 廖艳艳, 唐超, 蔡澎. 极低频电磁辐射增强秀丽线虫能量代谢并诱导氧化应激反应[J]. 生理学报 2019; 71 (3): 388-394.

SUN Yong-Yan, WANG Ya-Hong, LI Zhi-Hui, SHI Zhen-Hua, LIAO Yan-Yan, TANG Chao, CAI Peng. Extremely low frequency electromagnetic radiation enhanced energy metabolism and induced oxidative stress in Caenorhabditis elegans. Acta Physiol Sin 2019; 71 (3): 388-394 (in Chinese with English abstract).