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3α-雄烷二醇对戊四氮致痫大鼠的抗痫作用

张宇浩, 曹银祥, 马昱, 郭曦, 汪昕

复旦大学附属中山医院神经内科，上海 200032；复旦大学上海医学院生理与病理生理学系，上海 200032；复旦大学附属中山医院分部内科，上海 200052；复旦大学脑科学研究院，神经生物学国家重点实验室，上海 200032

摘要

本研究观察了3α-雄烷二醇(3α-androstanediol, 3α-diol)对戊四氮(pentylenetetrazole, PTZ)致痫大鼠抽搐发作及脑电图变化的影响，旨在从行为学及脑电图两方面的表现来探索3α-diol在癫痫发作中所起的作用。将32只雄性Sprague-Dawley大鼠随机均分成4组：正常+茶油+致痫 (N+oil+PTZ)组、正常+3α-diol+致痫(N+3α-diol+PTZ)组、阉割+茶油+致痫(GDX+oil+PTZ)组和阉割+3α-diol+致痫(GDX+3α-diol+PTZ)组，记录和分析各组大鼠行为学及脑电图的变化。行为学观察结果显示：GDX+oil+PTZ组较N+oil+PTZ组阵挛发作及强直-阵挛发作的潜伏期均明显缩短，强直-阵挛发作的次数明显增多(P < 0.05)；GDX+3α-diol+PTZ组与GDX+oil+PTZ组相比较，N+3α-diol+PTZ组与N+oil+PTZ组相比较，阵挛发作及强直-阵挛发作的潜伏期均明显延长，阵挛发作及强直-阵挛发作的次数均明显减少(P < 0.05)。脑电图分析结果显示：GDX+oil+PTZ组较N+oil+PTZ组痫波出现的潜伏期明显缩短， 痫波发放的次数明显增多(P < 0.05)；GDX+3α-diol+PTZ组与GDX+oil+PTZ组相比较，N+3α-diol +PTZ组与N+oil+PTZ组相比较，痫波出现的潜伏期明显延长，痫波发放的次数明显减少，脑电频谱总功率变化百分比(percentage of total power of spectrum, TP%)明显减小(P < 0.05)。这些结果提示，3α- diol对阉割和正常的致痫大鼠均具有一定的抗痫作用。

关键词： 癫痫; 3α-雄烷二醇; 行为学; 脑电图; 戊四氮

[The antiepileptic role of 3α-androstanediol on the epileptic rats induced by pentylenetetrazole.] [Article in Chinese]

ZHANG YuHao, CAO YinXiang, MA Yu, GUO Xi, WANG Xin

Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China； Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China； Department of Medicine, Branch of Zhongshan Hospital, Fudan University, Shanghai 200052, China； Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.

Abstract

The influence of 3α-androstanediol (3α-diol) on twitch and electroencephalogram (EEG) of the epileptic rats induced by pentylenetetrazole (PTZ) has been observed in this experiment in order to comprehensively explore the role of 3α-diol on epileptic attack from the aspects of behavior and EEG. Thirty-two male Sprague-Dawley rats were evenly and randomly divided into 4 groups: the normal and supplied with oil epileptic (N+oil+PTZ) group, the normal and supplied with 3α-diol epileptic (N+3α-diol+PTZ) group, the gonadectomized and supplied with oil epileptic (GDX+oil+PTZ) group and the gonadectomized and supplied with 3α-diol epileptic (GDX+3α-diol+PTZ) group. The changes of the behavior and EEG of epileptic rats in every group were recorded and analyzed. The results of behavior observation showed that the latency to clonic seizure and tonic-clonic seizure was shortened and the number of tonic-clonic seizure was increased significantly in the GDX+oil+PTZ group in comparison with N+oil+PTZ group (P < 0.05); comparing GDX+3α-diol+PTZ group with GDX+oil+PTZ group, or N+3α-diol+PTZ group with N+oil+PTZ group, we found that the latency to clonic seizure and tonic-clonic seizure became prolonged significantly, and the number of clonic seizure and tonic-clonic seizure was decreased significantly (P < 0.05). The results of EEG showed that the latency to epileptic waves was cut and the number of epileptic waves was augmented significantly in the GDX+oil+PTZ group in comparison with N+oil+PTZ group (P < 0.05); comparing GDX+3α-diol+PTZ group with GDX+oil+PTZ group, or N+3α-diol+PTZ group with N+oil+PTZ group, we found that the latency to epileptic waves became lengthened significantly, the number of epileptic waves was reduced significantly and the percentage of change of TP (total power of spectrum) was lessened significantly (P < 0.05). These results indicate that 3α-diol has an antiepileptic activity in the gonadectomized and normal epileptic rats.

Key words: Epilepsy; 3α-diol; behavior; EEG; pentylenetetrazole

收稿日期：2012-03-21　　录用日期：2012-09-24

通讯作者：汪昕　　E-mail: wang.xin@zs-hospital.sh.cn

引用本文：

张宇浩, 曹银祥, 马昱, 郭曦, 汪昕. 3α-雄烷二醇对戊四氮致痫大鼠的抗痫作用[J]. 生理学报 2012; 64 (6): 633-638.

ZHANG YuHao, CAO YinXiang, MA Yu, GUO Xi, WANG Xin. [The antiepileptic role of 3α-androstanediol on the epileptic rats induced by pentylenetetrazole.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (6): 633-638 (in Chinese with English abstract).