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模拟失重4周增加大鼠心肌的心房利钠肽表达

张文成, 卢元明, 杨怀章, 徐彭涛, 常惠, 余志斌

第四军医大学航空航天医学教育部重点实验室；航空航天生理教研室，西安 710032；空军西安飞行学院门诊部，西安 710306

摘要

失重或模拟失重引起体液的头向转移，导致上半身血容量增加，从而引起心房肌心房利钠肽(atrial natriuretic peptide, ANP)分泌的改变，但现有研究报道缺乏一致的结论。本研究拟观测模拟失重大鼠心肌ANP表达的变化，并以可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体(soluble N-ethylmaleimide-sensitive factor attachment protein receptors, SNARE)家族相关蛋白的变化作为ANP变化的佐证。采用公认的尾部悬吊大鼠模型在地面模拟失重状态，蛋白印迹法检测心房与心室肌ANP与SNARE相关蛋白的表达水平，并进行半定量分析。结果显示，与同步对照组(CON)相比，尾部悬吊4周大鼠(SUS)心房肌ANP表达增加；在CON大鼠左心室肌仅显示微量ANP表达，但SUS大鼠心室肌ANP表达明显增加。相比CON组，SUS组囊泡SNARE (v-SNARE) VAMP-1/2在心房肌与心室肌表达明显增加；细胞膜靶标SNARE (t-SNARE) syntaxin-4的表达未改变，但SNAP-23在心房肌的表达明显增加。SNARE调节蛋白synip与Munc-18c的表达在心房肌与心室肌未发生显著性改变。上述结果表明，尾部悬吊4周引起心房肌ANP表达增加，心室肌呈现ANP表达；与ANP囊泡相关的VAMP-1/2的表达亦增加，佐证心房肌与心室肌ANP表达增加。

关键词： 尾部悬吊模型; 大鼠; 心房利钠肽; 可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体

分类号：Q46

[Four-week simulated weightlessness increases the expression of atrial natriuretic peptide in the myocardium.] [Artilce in Chinese]

ZHANG Wen-Cheng, LU Yuan-Ming, YANG Huai-Zhang, XU Peng-Tao, CHANG Hui, YU Zhi-Bin

Key Laboratory of Aerospace Medicine, Ministry of Education, Department of Aerospace Physiology, the Fourth Military Medical University, Xi'an 710032, China； Clinic of Air Force Flying College, Xi'an 710306, Chin

Abstract

One of the major circulatory changes that occur in human during space flight and simulated weightlessness is a cerebral redistribution of body fluids, which is accompanied by an increase of blood volume in the upper body. Therefore, atrial myocardium should increase the secretion of atrial natriuretic peptide (ANP), but the researches lack common conclusion until now. The present study was to investigate the expression level of ANP in simulated weightlessness rats, and to confirm the changes of ANP by observing the associated proteins of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The tail-suspended rat model was used to simulate weightlessness. Western blots were carried out to examine the expression levels of ANP and SNARE proteins in atrial and left ventricular myocardium. The results showed that ANP expression in atrial myocardium showed an increase in 4-week tail-suspended rats (SUS) compared with that in the synchronous control rats (CON). We only detected a trace amount of ANP in the left ventricular myocardium of the CON, but found an enhanced expression of ANP in left ventricular myocardium of the SUS. Expression of VAMP-1/2 (vesicle associated SNARE) increased significantly in both atrial and left ventricular myocardium in the SUS compared with that in the CON. There was no difference of the expression of syntaxin-4 (target compartment associated SNARE) between the CON and SUS, but the expression of SNAP-23 showed an increase in atrial myocardium of the SUS compared with that in the CON. Synip and Munc-18c as regulators of SNAREs did not show significant difference between the CON and SUS. These results suggest that the expression of ANP shows an increase in atrial and left ventricular myocardium of 4-week tail-suspended rats. Enhanced expression of VAMP-1/2 associated with ANP vesicles confirms the increased expression of ANP in atrial and left ventricular myocardium.

Key words: tail-suspended model; rat; atrial natriuretic peptide; soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)

收稿日期：2012-06-22　　录用日期：2012-11-20

通讯作者：余志斌　　E-mail: yuzhib@fmmu.edu.cn

引用本文：

张文成, 卢元明, 杨怀章, 徐彭涛, 常惠, 余志斌. 模拟失重4周增加大鼠心肌的心房利钠肽表达[J]. 生理学报 2013; 65 (2): 143-148.

ZHANG Wen-Cheng, LU Yuan-Ming, YANG Huai-Zhang, XU Peng-Tao, CHANG Hui, YU Zhi-Bin. [Four-week simulated weightlessness increases the expression of atrial natriuretic peptide in the myocardium.] [Artilce in Chinese]. Acta Physiol Sin 2013; 65 (2): 143-148 (in Chinese with English abstract).