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自发性高血压大鼠和Wistar 大鼠淋巴细胞Kv 通道表达差异

罗健, 张源明, 马克涛, 司军强, 梁平

新疆医科大学第一附属医院 综合内科； 心脏中心，乌鲁木齐 830054； 石河子大学医学院生理学教研室，石河子 832000

摘要

本研究采用全细胞膜片钳技术、实时荧光定量 PCR 技术及Western blot 技术检测自发性高血压大鼠(spontaneously hypertensive rat, SHR)和Wistar 大鼠外周血淋巴细胞电压依赖性钾通道(voltage dependent potassium channel, Kv)电流密度及 Kv1.3 mRNA和蛋白表达水平，探讨淋巴细胞Kv 通道在高血压病中的变化，为高血压病淋巴细胞激活提供证据。结果显 示：(1) SHR 淋巴细胞 Kv 峰值(取自阶跃电压中+60 mV)电流密度为(119±10) pA/pF (n=30)，Wistar 大鼠为(56±9) pA/pF (n=40)，SHR 淋巴细胞Kv 峰值电流密度明显高于Wistar 大鼠(P<0.05); (2)与Wistar 大鼠相比，SHR 淋巴细胞Kv1.3 mRNA 表达增多(P<0.05); (3) SHR 淋巴细胞Kv1.3 蛋白表达水平高于Wistar 大鼠(P<0.05)。结果提示，SHR 淋巴细胞上有更多功 能性Kv 通道，Kv 通道可能与SHR 淋巴细胞激活有关。

关键词： 高血压病; 淋巴细胞; 钾通道

[Difference in the expression of Kv channel in lymphocytes between spontaneously hypertensive rats and Wistar rats.] [Ariticle in Chinese]

LUO Jian, ZHANG Yuan-Ming, MA Ke-Tao, SI Jun-Qiang, LIANG Ping

Department of Internal Medicine； Department of Cardiology, the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China； Department of Physiology, Shihezi Medical University, Shihezi 832000, China

Abstract

The present study aimed to investigate the difference in the voltage dependent potassium channel (Kv) expression in lymphocytes between the spontaneously hypertensive rats (SHRs) and Wistar rats. Peripheral blood lymphocytes were collected from 10 male SHRs and 10 normotensive Wistar rats aged 16 weeks. First, by using the patch-clamp technique, Kv channel current was recorded in freshly isolated lymphocytes from SHRs and normotensive Wistar rats. Total RNAs were extracted from lymphocytes by using TRIzol reagent. Real-time PCR was used to determine the expression of Kv1.3 mRNA and Western blot technique was used to measure the expression of Kv1.3 protein in lymphocytes from SHRs and normotensive Wistar rats. The results showed that: (1) The current density of Kv channel in the step voltage of +60 mV was higher in lymphocytes from SHRs than that from the normotensive Wistar rats [(119±10) pA/pF vs (56±9) pA/pF, P<0.05]; (2) The level of Kv1.3 mRNA expression in lymphocytes from SHRs was significantly increased compared with that of the normotensive Wistar rats (0.0313±0.017 vs 0.0023±0.005, P<0.05); (3) The expression of Kv1.3 protein was significantly elevated in lymphocytes (1.02±0.04 vs 0.41±0.03, P<0.05) from SHRs compared with that of the normotensive Wistar rats. The results obtained demonstrate that the lymphocytes Kv channels are increased in SHR, and the Kv channel may be involved in activation of lymphocytes from SHR.

Key words: hypertension; lymphocyte; potassium channel

收稿日期：2010-01-15　　录用日期：2010-03-29

通讯作者：张源明　　E-mail: zymdxx@163.com

引用本文：

罗健, 张源明, 马克涛, 司军强, 梁平. 自发性高血压大鼠和Wistar 大鼠淋巴细胞Kv 通道表达差异[J]. 生理学报 2010; 62 (4): 382-386.

LUO Jian, ZHANG Yuan-Ming, MA Ke-Tao, SI Jun-Qiang, LIANG Ping. [Difference in the expression of Kv channel in lymphocytes between spontaneously hypertensive rats and Wistar rats.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (4): 382-386 (in Chinese with English abstract).