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慢性阻塞性肺疾病大鼠海马区和血清中脑源性神经营养因子蛋白的表达变化

汪群, 林勇^*, 张蔷, 孙思庆, 凌学锋

东南大学医学院，南京 210009；东南大学附属中大医院呼吸科，南京 210009

摘要

本文旨在探讨脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)在慢性阻塞性肺疾病(chronic obstructive pulmonary disease, COPD)大鼠模型海马区和血清中的表达情况，以及了解吸烟和气管内注入脂多糖的干预是否参与BDNF表达变化。采用被动吸烟和气管中注入脂多糖建立大鼠COPD模型。将24只大鼠随机分为4组，每组各6只，分别为正常对照组、吸烟组、脂多糖组以及吸烟 + 脂多糖组(即COPD组)。造模28 d后，应用酶联免疫吸附实验(ELISA)方法测定各组大鼠血清中BDNF的表达水平，再通过免疫组化染色和图像分析方法半定量检测大鼠海马区BDNF的表达情况。结果显示，COPD组、吸烟组和脂多糖组血清及海马区BDNF表达水平均较正常组明显升高(P < 0.05)，且COPD组血清及海马区BDNF表达水平均较吸烟组和脂多糖组明显降低(P < 0.05)。以上结果提示，COPD大鼠海马区及血清中BDNF蛋白表达水平升高，且被动吸烟和气管内注入脂多糖均可诱导BDNF蛋白表达水平升高。

关键词： 慢性阻塞性肺疾病; 脑源性神经营养因子; 吸烟; 脂多糖; 认知功能

分类号：R563

[Changes of BDNF expression in hippocampus and serum of rats with artificial chronic obstructive pulmonary disease.] [Article in Chinese]

WANG Qun, LIN Yong^*, ZHANG Qiang, SUN Si-Qing, LING Xue-Feng

School of Medical College, Southeast University, Nanjing 210009, China； Respiratory Department, Zhongda Hospital, Southeast University, Nanjing 210009, China

Abstract

In clinical practice, we have found that cognitive impairment frequently occurs in chronic obstructive pulmonary disease (COPD) patients, but little is known about its pathophysiological mechanism. Given that brain-derived neurotrophic factor (BDNF) is affected by many factors such as smoking, infection, hypoperfusion and hypoxia, the present study was to explore the expression of BDNF in COPD rats. The rat COPD model was established by passive smoking and intratracheal instillation of lipopolysaccharide (LPS). Rats with the same age and gender ratios were divided into 4 groups: the control group (n = 6), the smoking group (n = 6), the LPS group (n = 6) and the smoking + LPS group (n = 6, COPD model). Level of BDNF in serum was measured by ELISA. And the expression of BDNF in the hippocampus was assessed using the immunohistochemistry and image analysis. The results showed that BDNF in the hippocampus and serum significantly increased in the smoking, LPS and smoking + LPS groups, compared to that in the control group. However, the expression of BDNF was less in the smoking + LPS group than that in the smoking or LPS group both in the hippocampus and serum. In conclusion, the expression of BDNF in the hippocampus and serum is highly increased in the COPD group. Smoking and intratracheal instillation of LPS induce the increase of BDNF level in the hippocampus and serum.

Key words: chronic obstructive pulmonary disease; brain-derived neurotrophic factor; smoking; lipopolysaccharide; cognitive function

收稿日期：2011-04-30　　录用日期：2011-07-26

通讯作者：林勇　　E-mail: linyong63@163.com

引用本文：

汪群, 林勇, 张蔷, 孙思庆, 凌学锋. 慢性阻塞性肺疾病大鼠海马区和血清中脑源性神经营养因子蛋白的表达变化[J]. 生理学报 2011; 63 (6): 505-510.

WANG Qun, LIN Yong, ZHANG Qiang, SUN Si-Qing, LING Xue-Feng. [Changes of BDNF expression in hippocampus and serum of rats with artificial chronic obstructive pulmonary disease.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (6): 505-510 (in Chinese with English abstract).