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地尔硫卓增强大鼠的摄食及胃消化功能

王丽娜, 李胜利, 李创宏, 张晨旭, 袁慧, 李新平^*

西北农林科技大学动物医学院，杨凌 712100；中国农业大学动物营养学国家重点实验室，北京 100083

摘要

本文旨在研究ghrelin受体激动剂地尔硫卓对大鼠摄食及胃肠功能的影响。实验通过给大鼠灌胃低、中、高浓度(每天16 mg/kg、30 mg/kg或80 mg/kg，30 d)的地尔硫卓溶液，灌胃等体积生理盐水为对照组，记录大鼠日均摄食量和日均体重增加量，运用放射免疫法测定大鼠血清中生长激素(growth hormone, GH)及神经肽Y (neuropeptide Y, NPY)的浓度，以检测地尔硫卓对大鼠摄食量及体重的影响；随后采用分光光度计测定光密度值并计算胃排空率，应用改良Mett氏法测定胃蛋白酶活性，用滴定法测大鼠胃游离酸和总酸度，计算胃酸总排出量，以检测地尔硫卓对大鼠胃肠功能的影响。结果显示，低、中、高浓度的地尔硫卓灌胃30 d对大鼠的摄食和体重的增加有一定的促进作用，大鼠平均每天的摄食量及体重与对照组相比显著增加，同时血清中GH及NPY的浓度也显著高于对照组，每天灌服剂量为30 mg/kg的地尔硫卓溶液时，促进作用最强；胃功能测定结果显示，与对照组相比，地尔硫卓处理组(每天灌服剂量为30 mg/kg)大鼠的胃排空率、胃酸分泌量及胃蛋白酶活性明显增加。以上结果表明，地尔硫卓对大鼠的摄食有一定的促进作用，同时对大鼠的胃肠道功能及生长有一定的调节作用。

关键词： 地尔硫卓; 摄食; 胃肠功能; ghrelin; 大鼠

分类号：S859.1；Q48

[Diltiazem enhances food intake and gastrointestinal function in rats.] [Article in Chinese]

WANG Li-Na, LI Sheng-Li, LI Chuang-Hong, ZHANG Chen-Xu, YUAN Hui, LI Xin-Ping^*

College of Veterinary Medicine, Northwest Agriculture and Forest University, Yangling 712100, China； State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100083, China

Abstract

The present study was to investigate the effects of diltiazem, a ghrelin receptor agonist, on food intake and gastrointestinal functions in rats. Rats were intragastrically administered with diltiazem solution (daily 16 mg/kg, 30 mg/kg or 80 mg/kg, 30 d), and the rats with saline as control. To detect the effects of diltiazem on food intake and body weight, the average daily food intake and body weight were recorded, and the serum metabolic hormones of plasma growth hormone (GH) and neuropeptide Y (NPY) were tested by radioimmunoassay. By means of the spectrophotometer and the modified Mett’s method, the effects of diltiazem on rat’s gastrointestinal function and pepsin activity were tested, respectively. In addition, the gastric juice’s acidity of rats was detected by titration and the secretion amount was calculated. The results showed that the food intake and body weight were maximally promoted by diltiazem at the dose of 30 mg/kg daily (30 d). The average daily food intake and body weight were significantly increased, and the serum concentrations of GH and NPY were also remarkably increased in diltiazem-treated groups compared with those in control group. The results also showed that the gastric emptying rate, gastric acid secretion and the activity of pepsin were significantly increased in diltiazem-treated group compared with those in control group. These results suggest that diltiazem induces enhancement of eating, in the same time, it can also stimulate the gastrointestinal function and regulate growth of rat.

Key words: diltiazem; food intake; gastrointestinal function; ghrelin; rats

收稿日期：2011-10-19　　录用日期：2011-12-20

通讯作者：李新平　　E-mail: lxp67cqu@163.com

引用本文：

王丽娜, 李胜利, 李创宏, 张晨旭, 袁慧, 李新平. 地尔硫卓增强大鼠的摄食及胃消化功能[J]. 生理学报 2012; 64 (2): 187-192.

WANG Li-Na, LI Sheng-Li, LI Chuang-Hong, ZHANG Chen-Xu, YUAN Hui, LI Xin-Ping. [Diltiazem enhances food intake and gastrointestinal function in rats.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (2): 187-192 (in Chinese with English abstract).