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白介素1β抑制培养的大鼠皮层神经元钠电流峰值和动作电位幅度

齐翠, 张伟伟, 李霄楠, 周辰^*

河北大学生命科学学院，保定 071002；河北大学基础医学院，保定 071000

摘要

白介素1β (interleukin-1β, IL-1β)是重要的促炎细胞因子，在中枢神经系统的生理学和病理学过程中发挥关键作用。电压门控钠通道是可兴奋细胞电学活动的基础，控制神经元的兴奋性和动作电位。最近的研究又显示了IL-1β与电压门控通道之间的相互作用。为考察中枢神经元中IL-1β与电压门控钠通道之间的相互作用，本研究使用10 ng/mL的IL-1β处理培养的大鼠皮层神经元24 h，通过电压钳技术测定电压门控钠电流，结果表明IL-1β处理抑制钠电流幅度，但不改变其激活和失活性质。与电压钳记录结果相一致，电流钳记录表明IL-1β降低动作电位幅度但不影响阈值。这些结果显示长时间的IL-1β处理可以抑制电压门控钠电流，这种抑制作用减小了动作电位幅度，这可能改变神经元的电学性质、突触传导等基本功能，并提示了IL-1β在神经系统损伤和疾病中作用的新的思路。

关键词： 白介素1β; 皮层神经元; 电压门控钠电流; 动作电位

分类号：Q424；Q426

[Interleukin-1β inhibits the amplitudes of voltage-gated Na(+) currents and action potential in cultured cortical neurons of rat.] [Article in Chinese]

QI Cui, ZHANG Wei-Wei, LI Xiao-Nan, ZHOU Chen^*

The College of Life Sciences, Hebei University, Baoding 071002, China； School of Basic Medical Science, Hebei University, Baoding 071000, China

Abstract

Interleukin-1β (IL-1β) is an important proinflammatory cytokine and plays key roles in physiological and pathophysiological processes of central nervous system (CNS). The voltage-gated Na+ channels are essential for electrical properties of excitable cells and control the excitability and action potential (AP) of neurons. Recent studies have showed the relationship between IL-1β and voltage-gated channels. In this work, cultured cortical neurons of rat were treated by 10 ng/mL of IL-1β for 24 h, and then voltage-gated Na+ currents were recorded using voltage-clamp technique. The results indicated that IL-1β reduced the amplitude of Na+ currents without any changes in activation or inactivation properties. The current-clamp recording showed that IL-1β reduced the amplitude of AP but not the threshold. These data indicate that IL-1β inhibits the voltage-gated Na+ currents and the amplitude of AP, and suggest that essential roles of voltage-gated Na+ channels may be changed by IL-1β. New information about effects of IL-1β on injuries and diseases of CNS was provided.

Key words: interleukin-1β; cortical neurons; voltage-gated Na+ currents; action potential

收稿日期：2010-11-25　　录用日期：2011-01-21

通讯作者：周辰　　E-mail: zezhou@sina.com

引用本文：

齐翠, 张伟伟, 李霄楠, 周辰. 白介素1β抑制培养的大鼠皮层神经元钠电流峰值和动作电位幅度[J]. 生理学报 2011; 63 (2): 131-137.

QI Cui, ZHANG Wei-Wei, LI Xiao-Nan, ZHOU Chen. [Interleukin-1β inhibits the amplitudes of voltage-gated Na(+) currents and action potential in cultured cortical neurons of rat.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (2): 131-137 (in Chinese with English abstract).