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雌激素受体GPR30 在上皮性卵巢癌中的表达及其与MMP-9表达的相关性

刘慧迪, 闫彦, 曹雪峰, 谭培珠, 温海霞, 吕春梅, 李晓梅, 刘国艺

哈尔滨医科大学 生理学教研室，哈尔滨 150081； 附属第三医院病理科，哈尔滨 150040

摘要

本文旨在研究新型雌激素受体——G蛋白耦联受体30 (G protein-coupled receptor 30, GPR30)在上皮性卵巢癌中的表 达，及其与基质金属蛋白酶9 (matrix metalloproteinases-9, MMP-9)表达的相关性。收集39 例上皮性卵巢肿瘤组织标本， 包括上皮性卵巢癌30 例(浆液性囊腺癌19 例、粘液性囊腺癌5 例和子宫内膜样癌6 例)、良性上皮性卵巢肿瘤9 例，并取4 例正常卵巢组织进行对照，应用免疫组织化学法检测GPR30 和MMP-9 的表达，使用2 检验、Fisher’s 精确检验和Spearman 等级相关分析等统计学方法对所得数据进行统计分析。结果显示，上皮性卵巢癌中GPR30 的高表达率显著高于良性卵巢肿 瘤(P<0.01)和正常卵巢组织(P<0.05) ；上皮性卵巢癌中MMP-9 的高表达率显著高于正常卵巢组织(P<0.05)，但与良性卵巢 肿瘤无明显差异。肿瘤病理类型、分期和患者年龄因素与GPR30 高表达率的相关性分析显示：不同病理类型上皮性卵巢 癌的GPR30 高表达率之间存在显著性差异 (P<0.05)，而各病理类型的MMP-9 高表达率之间没有差异；临床III~IV 期上皮 性卵巢癌的GPR30 高表达率显著高于I~II 期(P<0.05)，二者MMP-9 高表达率之间没有差异；三个年龄组病例中的GPR30 高表达率之间未见明显差异，三者MMP-9 高表达率之间亦没有差异。Spearman 等级相关分析显示上皮性卵巢癌组织中 GPR30 与MMP-9 的表达之间具有正向相关性(P<0.01)。以上结果提示，GPR30 可能参与上皮性卵巢癌的发生发展，并与 其侵袭和转移具有一定的相关性，可作为卵巢癌早期诊断和恶性程度判断的指标之一。

关键词： 上皮性卵巢癌; G 蛋白耦联受体3 0 ; 基质金属蛋白酶9

分类号：R737.31

[The expression of a novel estrogen receptor, GPR30, in epithelial ovarian carcinoma and its correlation with MMP-9.] [Ariticle in Chinese]

LIU Hui-Di, YAN Yan, CAO Xue-Feng, TAN Pei-Zhu, WEN Hai-Xia, LV Chun-Mei, LI Xiao-Mei, LIU Guo-Yi

Department of Physiology, Harbin Medical University, Harbin 150081, China； Department of Pathology, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, China

Abstract

The aim of the present study is to investigate the expression of a novel estrogen receptor, G protein-coupled receptor 30 (GPR30) and its correlation with matrix metalloproteinases-9 (MMP-9) in epithelial ovarian cancer (EOC). Ovary tissues were obtained from 39 female patients, including 30 cases of EOC and 9 cases of benign ovarian tumor. Four normal ovary tissues were used as control. Immunohistochemical staining was used to detect the expressions of GPR30 and MMP-9. Chi square test, Fisher’s exact test and Spearman’s rank correlation analysis were used for statistical analysis. The results showed that GPR30 overexpression rate in EOC cases was significantly higher than those in benign ovarian tumor and normal ovary cases. Whereas MMP-9 overexpression rate in EOC cases was significantly higher than that in normal ovary cases, without any difference to that in benign ovarian tumor cases. To demonstrate the relationship between GPR30 and clinicopathological variables of EOC, we further analyzed the pathology type, FIGO stage and age of patients sampled in our study. The analysis showed there were significant differences of GPR30 overexpression rate among various pathology types and different FIGO stages (P<0.05), and no significant difference of both GPR30 and MMP-9 among three age groups (P>0.05). Moreover, GPR30 expression was positively correlated with MMP-9 (rs=1.000, P=0.002). These results suggest that GPR30 may be involved in the invasion and metastasis of EOC, being a potential index of EOC early diagnosis and malignancy grade prediction.

Key words: epithelial ovarian carcinoma; G protein-coupled receptor 30; matrix metalloproteinases-9

收稿日期：2010-06-02　　录用日期：2010-07-02

通讯作者：刘国艺　　E-mail: liuguoyi@126.com

引用本文：

刘慧迪, 闫彦, 曹雪峰, 谭培珠, 温海霞, 吕春梅, 李晓梅, 刘国艺. 雌激素受体GPR30 在上皮性卵巢癌中的表达及其与MMP-9表达的相关性[J]. 生理学报 2010; 62 (6): 524-528.

LIU Hui-Di, YAN Yan, CAO Xue-Feng, TAN Pei-Zhu, WEN Hai-Xia, LV Chun-Mei, LI Xiao-Mei, LIU Guo-Yi. [The expression of a novel estrogen receptor, GPR30, in epithelial ovarian carcinoma and its correlation with MMP-9.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (6): 524-528 (in Chinese with English abstract).