电针诱导心肌缺血大鼠延髓头端腹外侧区nNOS和iNOS差异表达
夏春梅, 陈军, 王锦, 樊明欣, 肖芬, 曹银祥, 李莉, 沈霖霖, 朱大年
复旦大学上海医学院生理与病理生理学系.上海 200032
摘要
许多研究表明,延髓头端腹外侧区(rostral ventrolateral medulla,RVLM)的NO/NOS系统参与心血管活动的中枢调节。该实验以结扎Wistar大鼠左冠状动脉前降支法建立急性心肌缺血(acute myocardial ischemia,AMI)动物模型,观察针刺“内关”穴改善AMI大鼠的心功能作用,同时检测大鼠RVLM区神经元型一氧化氮合酶(neuronal nitri coxide synthase,nNOS)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)表达的变化,进而探讨针刺治疗AMI的中枢机制。实验观察显示,AMI大鼠心功能各项指标减弱,伴随外周血去甲肾上腺素(norepinephrine,NE)和脑钠肽(brain natriuretic peptide,BNP)水平显著升高,同时RVLM区nNOS阳性神经元数和nNOSmRNA表达升高,而iNOS水平则降低。针刺“内关”穴(Pe 6)(每天30min,连续5d)改善心功能,降低AMI大鼠血清中NE和BNP的水平,同时升高iNOS并降低nNOS在RVLM的表达。以上结果提示,针刺治疗心肌缺血的同时可以调节iNOS/NO和nNOS/NO在RVLM的变化,这可能与针刺通过调节RVLM区的NO含量进而降低交感传出,从而改善AMI大鼠的心功能有关。
关键词: 延髓头端腹外侧区; 心肌缺血; 电针; 神经元型一氧化氮合酶; 诱导型一氧化氮合酶
Differential expressions of nNOS and iNOS in the rostral ventrolateral medulla induced by electroacupuncture in acute myocardial ischemia rats
Xia Chunmei, Chen Jun, Wang Jin, Fan Mingxin, Xiao Fen, Cao Yinxiang, Li Li, Shen Linlin, Zhu Danian
Department of Physiology and Pathophysiology,Shanghai Medical College of Fudan University.Shanghai 200032
Abstract
Increasing lines of evidence has been accumulated that nitric oxide (NO) and nitric oxide synthase (NOS) distribute plentifully in the rostral ventrolateral medulla (RVLM) and contribute to cardiovascular regulation. In the present study, the expressions of neuronal and inducible isoform of NOS (nNOS and iNOS) were observed in the RVLM of acute myocardial ischemia (AMI) Wistar rats experienced electroacupuncture (EA) treatment, thereby the cardiovascular effects of NO in the RVLM were investigated and the mechanism of acupuncture effect on AMI was inferred. The results indicated that in the AMI rats, cardiac functions were markedly attenuated with high serum level of brain natriuretic peptide (BNP) and norepinephine (NE), the number of nNOS-immunoreactive cells and nNOS mRNA exprossion in the RVLM area were increased, while those of iNOS were lowered. EA at "Neiguan" acupoints (Pe 6) 30 min daily for successive 5 d resulted in an improvement of the cardiac functions, decreases in NE and BNP levels; it also increased the expression of iNOS and decreased the expression of nNOS in the RVLM. These results suggest that the curative effect of acupuncture on AMI is possibly attributable to the differential regulation of NOS/NO in the RVLM, leading to decreased sympathetic outflow and improvement of cardiac functions.
Key words: Rostral ventrolateral medulla;Myocardial ischemia;Electroacupuncture;Neuronal nitric oxide synthase;Inducible nitric oxide synthase
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夏春梅, 陈军, 王锦, 樊明欣, 肖芬, 曹银祥, 李莉, 沈霖霖, 朱大年. 电针诱导心肌缺血大鼠延髓头端腹外侧区nNOS和iNOS差异表达[J]. 生理学报 2008; 60 (4): 453-461.
Xia Chunmei, Chen Jun, Wang Jin, Fan Mingxin, Xiao Fen, Cao Yinxiang, Li Li, Shen Linlin, Zhu Danian. Differential expressions of nNOS and iNOS in the rostral ventrolateral medulla induced by electroacupuncture in acute myocardial ischemia rats. Acta Physiol Sin 2008; 60 (4): 453-461 (in Chinese with English abstract).