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双侧杏仁核注射降钙素基因相关肽对小鼠学习记忆的作用

武鑫, 郑婉君, 吕明惠, 高剑峰^*

河南中医药大学基础医学院生理学学科，郑州 450008

摘要

本研究旨在探讨外源性给予杏仁核中央核区不同浓度的降钙素基因相关肽(calcitonin gene-related peptide, CGRP)对小鼠认知和学习记忆的影响。将30日龄C57BL/6J小鼠随机分为空白对照组、手术对照组、200 ng CGRP组、400 ng CGRP组和800 ng CGRP组，每组10只。外源性给予双侧杏仁核中央核区不同浓度的CGRP后，用旷场实验检测各组小鼠认知功能，用新物体认知实验和Morris水迷宫实验检测各组小鼠学习记忆能力。旷场实验结果显示，与空白对照组相比，800 ng CGRP组小鼠的水平运动得分显著增加。新物体认知实验结果显示，与空白对照组相比，400 ng CGRP组的认知指数显著提高。Morris水迷宫实验结果显示，与空白对照组比较，400和800 ng CGRP组小鼠的潜伏期均显著缩短，穿越平台的次数显著增加；与200 ng CGRP组比较，400和800 ng CGRP组小鼠寻找平台的潜伏期均明显缩短。以上结果提示，双侧杏仁核注射CGRP可剂量依赖性促进小鼠非空间和空间学习记忆功能。

关键词： 降钙素基因相关肽; 杏仁核中央核区 ; 学习记忆 ; 突触可塑性

分类号：R338.64

Effect of bilateral injection of calcitonin gene-related peptide into amygdala on learning and memory of mice

WU Xin, ZHENG Wan-Jun, LV Ming-Hui, GAO Jian-Feng^*

Department of Physiology, School of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China

Abstract

The aim of the present study was to explore the effects of different doses of calcitonin gene-related peptide (CGRP) injected into the central nucleus of amygdala on cognitive function, learning and memory of mice. C57BL/6J mice (30 days old) were randomly divided into control, sham, and three CGRP groups (10 mice for each group). Three doses of CGRP (200, 400 and 800 ng) were bilaterally administered into the central nucleus of the amygdala. Open field test was used to assess cognitive function. Novel object recognition and Morris water maze test were used to evaluate learning and memory of the mice. The results of open field test showed that 800 ng CGRP significantly increased the locomotive score. The results of novel objective recognition test showed that 400 ng CGRP significantly increased the recognition index. Compared with control group, 400 and 800 ng CGRP groups showed significantly shortened latency period and increased crossing times. Simultaneously, the latency periods of 400 and 800 ng CGRP groups were shorter than that of 200 ng CGRP group. These results suggest that bilateral injection of CGRP into amygdala dose- dependently enhances the learning and memory function of mice.

Key words: Calcitonin gene-related peptide; central nucleus of amygdala ; learning and memory ; synaptic plasticity

收稿日期：2016-08-30　　录用日期：2016-10-17

通讯作者：高剑峰　　E-mail: jc.gjfeng@hotmail.com

引用本文：

武鑫, 郑婉君, 吕明惠, 高剑峰. 双侧杏仁核注射降钙素基因相关肽对小鼠学习记忆的作用[J]. 生理学报 2017; 69 (2): 167-171.

WU Xin, ZHENG Wan-Jun, LV Ming-Hui, GAO Jian-Feng. Effect of bilateral injection of calcitonin gene-related peptide into amygdala on learning and memory of mice. Acta Physiol Sin 2017; 69 (2): 167-171 (in Chinese with English abstract).