ISSN 0371-0874, CN 31-1352/Q

过刊浏览

淫羊藿苷减轻MPP+对MES23.5细胞的神经毒性损伤

徐爱丽, 姜明春, 陈筱涵, 陈文芳

青岛大学医学院生理学教研室,山东省神经退变疾病协同创新中心,青岛 266071;滨州医学院生理学教研室,烟台 264003;泰山医学院生理学教研室,泰安 271016

摘要

淫羊藿苷是传统中药淫羊藿的主要活性成分。研究显示淫羊藿苷可能是一个潜在的抗老年疾病的药物。本研究旨在探讨淫羊藿苷对MPP+诱导的MES23.5细胞损伤的保护作用及其可能机制。细胞活力检测结果显示MPP+可剂量依赖性地损伤MES23.5细胞,降低细胞存活率,淫羊藿苷可以显著抑制MPP+所诱导的细胞毒性作用。此外,免疫细胞化学和免疫印迹结果显示淫羊藿苷可对抗MPP+引起的酪氨酸羟化酶(TH)阳性细胞数的减少和蛋白水平的降低。流式细胞术结果显示,淫羊藿苷可以逆转MPP+所导致的线粒体膜电位的降低。Real-time RT-PCR和Western blot结果显示,淫羊藿苷可逆转MPP+所导致的Bax基因和蛋白表达水平的上调以及Bcl-2基因和蛋白表达水平的下调;同时,淫羊藿苷也可以抑制MPP+所诱导的cleaved caspase-3蛋白的表达水平。以上结果提示,淫羊藿苷可明显对抗MPP+对MES23.5细胞的神经毒性作用,其作用机制可能与抗凋亡有关。

关键词: 淫羊藿苷; 1-甲基-4-苯吡啶离子; MES23.5细胞

分类号:R338

Icariin protects against MPP+-induced neurotoxicity in MES23.5 cells

XU Ai-Li, JIANG Ming-Chun, CHEN Xiao-Han, CHEN Wen-Fang

Department of Physiology, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Medical College of Qingdao University, Qingdao 266071, China; Department of Physiology, Binzhou Medical University, Yantai 264003, China; Department of Physiology, Medical College of Taishan, Taian 271016, China

Abstract

Icariin is the major bioactive component of Epimedium and has been demonstrated to be a potential drug for age-related diseases. The present study was aimed to investigate the neuroprotective properties of icariin against 1-methyl-4-phenylpyridinium ion (MPP+)-induced neurotoxicity in MES23.5 cells and the possible mechanisms. MTT assay showed that treatment with MPP+ attenuated the cell viability in a dose-dependent manner in MES23.5 cells. Icariin pretreatment resulted in an enhancement of survival. Immunocytochemistry analysis revealed that icariin treatment attenuated MPP+-induced loss of tyrosine hydroxylase (TH) positive cells. Meanwhile, Western blot confirmed MPP+ significantly decreased the TH protein expression, and icariin pretreatment could reverse the toxic effect of MPP+. Moreover, flow cytometry showed that MPP+-induced decrease of the mitochondrial membrane potential could be partly restored by icariin. Furthermore, real-time RT-PCR and Western blot analysis demonstrated that icariin treatment restored the MPP+-induced up-regulation of Bax and down-regulation of Bcl-2 mRNA and protein expressions. Western blot data also revealed the inhibitory effect of icariin on MPP+-induced up-regulation of cleaved caspase-3. These findings provide the evidence that icariin has neuroprotective properties against MPP+-induced neurotoxicity in MES23.5 cells and the mechanism might be related to the anti-apoptotic action of icariin.

Key words: icariin; 1-methyl-4-phenylpyridinium ion; MES23.5 cells

收稿日期:2016-02-25  录用日期:2016-07-20

通讯作者:陈文芳  E-mail: chenwenfangqd@163.com

引用本文:

徐爱丽, 姜明春, 陈筱涵, 陈文芳. 淫羊藿苷减轻MPP+对MES23.5细胞的神经毒性损伤[J]. 生理学报 2016; 68 (5): 585-591.

XU Ai-Li, JIANG Ming-Chun, CHEN Xiao-Han, CHEN Wen-Fang. Icariin protects against MPP+-induced neurotoxicity in MES23.5 cells. Acta Physiol Sin 2016; 68 (5): 585-591 (in Chinese with English abstract).