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乙酰胆碱在钩吻素己致小鼠中毒死亡中的作用

赖周毅, 王海波, 吕瑞玲, 谭秋婵, 邓志钦, 汪源, 孙晓雪, 伍嘉宝, 朱林燕, 王磊, 陈丽新, 叶文才, 王立伟^*

暨南大学1医学院生理学系；2医学院药理学系；3药学院，广州 510632

摘要

本文旨在探讨全血乙酰胆碱浓度与钩吻素己致昆明小鼠中毒死亡的关系。给昆明小鼠腹腔注射生理盐水、钩吻素己或不同剂量的氯化乙酰胆碱溶液，并对注射钩吻素己和中剂量氯化乙酰胆碱的小鼠给予阿托品解救。中毒死亡小鼠死后即时取血；存活小鼠在注射药液后20 min处死取血。用试剂盒测定小鼠全血乙酰胆碱浓度与乙酰胆碱酯酶活性，计算各组死亡率并分析其死亡情况。结果显示，相对于生理盐水对照组，腹腔注射半数致死量(0.15 mg/kg)的钩吻素己后，小鼠全血乙酰胆碱酯酶活力降低，全血乙酰胆碱水平升高，但其中死亡小鼠的全血乙酰胆碱浓度显著低于中剂量氯化乙酰胆碱组死亡小鼠的水平，而与中剂量乙酰胆碱组存活小鼠的水平无差异。阿托品可有效解救氯化乙酰胆碱中毒小鼠，但对钩吻素己中毒小鼠的抢救效果不明显。以上结果提示，钩吻素己可引起小鼠全血胆碱酯酶活力下降，乙酰胆碱浓度升高，但体内乙酰胆碱堆积可能并不是钩吻素己致小鼠中毒死亡的主要或唯一原因。

关键词： 昆明小鼠; 钩吻素己; 乙酰胆碱; 乙酰胆碱酯酶

Role of acetylcholine in gelsenicine-induced death in mice

LAI Zhou-Yi, WANG Hai-Bo, LV Rui-Ling, TAN Qiu-Chan, DENG Zhi-Qin, WANG Yuan, SUN Xiao-Xue, WU Jia-Bao, ZHU Lin-Yan, WANG Lei, CHEN Li-Xin, YE Wen-Cai, WANG Li-Wei^*

1Department of Physiology； 2Department of Pharmacology, Medical College； 3College of Pharmacy, Jinan University, Guangzhou 510632, China

Abstract

The aim of this study was to investigate the relationship between the acetylcholine concentration in the blood and gelsenicine-induced death in mice. Kunming mice were given intraperitoneal injections of normal saline, gelsenicine or different doses of
acetylcholine chloride. Atropine was given to the mice which received gelsenicine or medium dose acetylcholine chloride injection.The blood was sampled immediately when the mice died or survived for 20 min after injection. The acetylcholine concentration and acetylcholinesterase activity in the blood were measured by the testing kits, and the mortality was calculated and analyzed. The results showed that half lethal dose of gelsenicine (0.15 mg/kg) reduced the acetylcholinesterase activity and increased the blood acetylcholine concentration. The blood acetylcholine concentration of the dead mice in the gelsenicine group was increased to 43.0 μg/mL (from 31.1 μg/mL in the control), which was lower than that (53.9 μg/mL) of the dead mice in the medium dose acetylcholine chloride group, but almost equal to that (42.7 μg/mL) of the survival mice in the medium dose acetylcholine chloride group. Atropine could successfully rescue the mice from acetylcholine poisoning, but its efficiency of rescuing the mice from gelsenicine intoxication was weak. These results suggest that gelsenicine can inhibit acetylcholinesterase activity and increase blood acetylcholine concentration,
but the accumulation of acetylcholine may not be the only or main cause of the death induced by gelsenicine in mice.

Key words: Kunming mice; gelsenicine; acetylcholine; acetylcholinesterase

收稿日期：　　录用日期：

通讯作者：王立伟　　E-mail: twangliwei@jnu.edu.cn

引用本文：

赖周毅, 王海波, 吕瑞玲, 谭秋婵, 邓志钦, 汪源, 孙晓雪, 伍嘉宝, 朱林燕, 王磊, 陈丽新, 叶文才, 王立伟. 乙酰胆碱在钩吻素己致小鼠中毒死亡中的作用[J]. 生理学报 2016; 68 (3): 249-254.

LAI Zhou-Yi, WANG Hai-Bo, LV Rui-Ling, TAN Qiu-Chan, DENG Zhi-Qin, WANG Yuan, SUN Xiao-Xue, WU Jia-Bao, ZHU Lin-Yan, WANG Lei, CHEN Li-Xin, YE Wen-Cai, WANG Li-Wei. Role of acetylcholine in gelsenicine-induced death in mice. Acta Physiol Sin 2016; 68 (3): 249-254 (in Chinese with English abstract).