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急性冷应激大鼠肝脏中mir-210的生物学功能预测

郭文晋, 连帅, 郭景茹, 翟骏飞, 张宇辰, 李悦, 甄莉, 计红, 杨焕民^*

黑龙江八一农垦大学动物科技学院，大庆 163000

摘要

本文通过观察冷应激时mir-210在大鼠肝脏中的表达情况，预测了mir-210在冷应激中的功能。本实验采用了30只12周龄SPF级Wistar雄性大鼠，体重(340 ± 20) g，将大鼠在常温预饲一周后，随机分成急性冷应激组[温度(4 ± 0.1) °C]和常温对照组[温度(24 ± 0.1) °C]，急性应激12 h后，提取肝脏，qRT-PCR检测mir-210表达。结果表明，急性冷应激组大鼠肝脏中的mir-210表达量升高，与常温对照组相比差异极显著(P < 0.01)。通过生物信息学方法分析发现，mir-210的靶基因有上百种，其中与冷应激相关性较高的靶基因是E2F3、RAD52、ISCU和Ephrin-A3。ISCU能够调节细胞的呼吸代谢，而Ephrin-A3对细胞的增殖和凋亡有显著影响；mir-210的上调还能够直接影响DNA的修复机制，从而导致遗传不稳定。结果提示，冷应激时肝脏mir-210的上调能够对细胞生长发育、能量代谢和遗传等方面产生影响。

关键词： 冷应激; mir-210; 肝脏; qRT-PCR; 生物信息学

分类号：Q494

[Biological function prediction of mir-210 in the liver of acute cold stress rat.] [Article in Chinese]

GUO Wen-Jin, LIAN Shuai, GUO Jing-Ru, ZHAI Jun-Fei, ZHANG Yu-Chen, LI Yue, ZHEN Li, JI Hong, YANG Huan-Min^*

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163000, China

Abstract

The study was aimed to observe mir-210 expression in liver tissue of acute cold stress rat and predict the function of mir-210 in cold stress. Thirty SPF Wistar male rats which were 12-week-old and weighed (340 ± 20) g were used. The rats were pre-fed in normal room temperature for one week, and then were randomly divided into acute cold stress group at (4 ± 0.1) °C and normal control group at (24 ± 0.1) °C. After the rats were treated with cold stress for 12 h, the liver tissue was extracted and the gene expression of mir-210 was assayed using qRT-PCR. The results demonstrated that the gene expression of mir-210 was significantly enhanced in acute cold stress group compared with that in normal control group (n = 3, P < 0.01). The bioinformatics analysis showed that mir-210 has over hundreds of target genes and four kinds of target genes such as E2F3, RAD52, ISCU and Ephrin-A3 are more relative with liver cold stress. ISCU regulates the cell respiratory metabolism and Ephrin-A3 is related with cell proliferation and apoptosis. On the other hand, up-regulated mir-210 affects the DNA repairing mechanism which usually leads to genetic instabilities. Our results suggest that cold stress-induced up-regulation of mir-210 in liver harmfully influences cell growth, energy metabolism and hereditary.

Key words: cold stress; liver; mir-210; qRT-PCR; bioinformatics analysis

收稿日期：2015-12-17　　录用日期：2016-03-01

通讯作者：杨焕民　　E-mail: yanghuanmin@aliyun.com

引用本文：

郭文晋, 连帅, 郭景茹, 翟骏飞, 张宇辰, 李悦, 甄莉, 计红, 杨焕民. 急性冷应激大鼠肝脏中mir-210的生物学功能预测[J]. 生理学报 2016; 68 (2): 165-170.

GUO Wen-Jin, LIAN Shuai, GUO Jing-Ru, ZHAI Jun-Fei, ZHANG Yu-Chen, LI Yue, ZHEN Li, JI Hong, YANG Huan-Min. [Biological function prediction of mir-210 in the liver of acute cold stress rat.] [Article in Chinese]. Acta Physiol Sin 2016; 68 (2): 165-170 (in Chinese with English abstract).