过刊浏览

触液核神经元蛋白激酶C磷酸化水平调制炎性痛大鼠的痛觉

周芳, 王家友, 田恩琪, 张励才

徐州医学院护理学院；江苏省麻醉学重点实验室，徐州 221004；安徽医科大学第二附属医院麻醉科，合肥 230000；山西省长治市人民医院麻醉科，长治 046000

摘要

本研究旨在探讨触液核在炎性疼痛中的作用及其机制。用大鼠左侧足底皮下注射完全弗氏佐剂(complete Freund’s adjuvant, CFA)来建立炎性痛模型，用Western blot检测炎性痛模型大鼠触液核中蛋白激酶C (protein kinase C, PKC)磷酸化水平的变化，用热缩足反射潜伏期(thermal withdrawal latency, TWL)检测来了解大鼠炎性痛觉情况。结果显示，炎性痛大鼠CFA注射后第1、3和7天TWL显著降低，注射CFA后24 h时触液核中p-PKC蛋白水平显著高于正常对照大鼠；侧脑室注射PKC抑制剂GF109203X可降低炎性痛大鼠触液核PKC磷酸化水平并提高痛觉阈值。以上结果提示，阻断触液核中PKC通路的信号转导可能是减轻或消除炎性痛的有效手段。

关键词： 液核; 蛋白激酶C; 炎性痛

分类号：Q189

[Phosphorylation of protein kinase C in cerebrospinal fluid-contacting nucleus modulates the inflammatory pain in rats.] [Article in Chinese]

ZHOU Fang, WANG Jia-You, TIAN En-Qi, ZHANG Li-Cai

School of Nursing； Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China； Anesthesiology Department of the Second Affiliated Hospital of Anhui Medical University, Hefei 230000, China； Department of Anesthesiology, the People’s Hospital of Changzhi, Changzhi 046000, China

Abstract

The present study was aimed to investigate the role of cerebrospinal fluid-contacting nucleus (CSF-CN) neurons in modulation of inflammatory pain and underlying mechanism. The inflammatory pain model was made by subcutaneous injection of the complete Freund’s adjuvant (CFA) into the left hind paw of rats. The phosphorylation level of PKC (p-PKC) was examined by Western blot. Thermal withdrawal latency (TWL) of the rats was measured to assess inflammatory pain. The results showed that, compared with the sham controls, the inflammatory pain model rats showed shortened TWL on day 1, 3, and 7 after CFA injection, as well as increased level of p-PKC in CSF-CN neurons at 24 h after CFA injection. The administration of GF109203X, a PKC inhibitor, into lateral ventricle decreased the level of p-PKC protein expression and increased TWL in the model rats. These results suggest that blocking the PKC pathway in CSF-CN neurons may be an effective way to reduce or eliminate the inflammatory pain.

Key words: CSF-CN; protein kinase C; inflammatory pain

收稿日期：2015-07-30　　录用日期：2015-10-27

通讯作者：张励才　　E-mail: licaizhang001@163.com

引用本文：

周芳, 王家友, 田恩琪, 张励才. 触液核神经元蛋白激酶C磷酸化水平调制炎性痛大鼠的痛觉[J]. 生理学报 2015; 67 (6): 591-595.

ZHOU Fang, WANG Jia-You, TIAN En-Qi, ZHANG Li-Cai. [Phosphorylation of protein kinase C in cerebrospinal fluid-contacting nucleus modulates the inflammatory pain in rats.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (6): 591-595 (in Chinese with English abstract).