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雷公藤红素抑制食管癌细胞转移及其机制

徐佳, 伍春莲

西华师范大学西南野生动植物资源保护省部共建教育部重点实验室，南充 637009；西南大学三峡库区生态环境与生物资源省部共建国家重点实验室，重庆 400715

摘要

雷公藤红素是从传统中药雷公藤中提取分离得到的一种醌甲基三萜，具有抗炎、免疫抑制及抗肿瘤等药理活性。本文探讨雷公藤红素对食管癌细胞粘附、迁移及侵袭的影响。用MTT法检测不同浓度的雷公藤红素对食管癌细胞ECA-109粘附的影响；用划痕实验测定不同浓度雷公藤红素对ECA-109细胞迁移的影响；用Transwell实验测定不同浓度雷公藤红素对ECA-109细胞侵袭的影响；用实时荧光定量PCR及Western blot实验检测不同浓度的雷公藤红素对ECA-109中整联蛋白家族及Wnt信号通路的影响。结果显示：雷公藤红素以剂量依赖的方式抑制ECA-109的粘附、迁移及侵袭，抑制整联蛋白β1、β4、αv基因和蛋白的表达，并抑制Wnt信号通路中β-Catenin、LRP6蛋白的表达。研究结果表明雷公藤红素能通过抑制Wnt信号通路和整联蛋白的表达来抑制食管癌细胞的转移。

关键词： 雷公藤红素; 食管癌; 转移; 整联蛋白; Wnt信号通路

分类号：R285

[Anti-metastasis of celastrol on esophageal cancer cells and its mechanism.] [Article in Chinese]

XU Jia, WU Chun-Lian

1Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong 637009, China； 2State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, Southwest University, Chongqing 400715, China

Abstract

Celastrol is a quinone methyl terpene extracted from the traditional Chinese medicine Tripterygium wilfordii, which has anti-inflammatory, immune suppression and pharmacological activities, as well as anti-tumor activity. The effects of celastrol on adhesion, migration and invasion of esophageal cancer cells were investigated in this experiment. Human esophageal cancer cell line ECA-109 was used. The inhibition of ECA-109 cells’ adhesion induced by celastrol was measured by MTT test. The inhibition of ECA-109 cells’ migration induced by celastrol was measured by scratch test. The invasion inhibition of ECA-109 cells induced by celastrol was measured by Transwell experiment. Quantitative real-time PCR and Western blot were used to determine the effects of different concentrations of celastrol on integrin family and Wnt signaling pathway in ECA-109 cells. The results showed that celastrol inhibited adhesion, migration and invasion of ECA-109 cells and expressions of integrins β1, β4, αv and β-Catenin, LRP6 in Wnt signal pathway in a dose-dependent manner. Therefore the study suggests that celastrol could inhibit the cell metastasis of esophageal cancer by inhibiting the Wnt signaling pathway and the expressions of integrins.

Key words: celastrol; esophageal cancer; metastasis; integrins; Wnt signal pathway

收稿日期：2014-12-31　　录用日期：2015-03-25

通讯作者：伍春莲　　E-mail: wcl_xj@163.com

引用本文：

徐佳, 伍春莲. 雷公藤红素抑制食管癌细胞转移及其机制[J]. 生理学报 2015; 67 (3): 341-347.

XU Jia, WU Chun-Lian. [Anti-metastasis of celastrol on esophageal cancer cells and its mechanism.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (3): 341-347 (in Chinese with English abstract).