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日节律紊乱对大鼠妊娠及子代的影响

陈文俊, 盛文洁, 郭银华, 谈勇^*

南京中医药大学第一临床医学院，南京 210023

摘要

本研究利用持续光照大鼠模型，观察日节律紊乱对大鼠妊娠及其子代早期的生长发育的影响，并初步探讨其与褪黑素及孕酮变化的关系。妊娠期给予24 h持续光照，观察日节律紊乱对大鼠分娩的影响。大鼠受孕后，在不同妊娠阶段采集血清，检测褪黑素和孕酮水平。在妊娠15日龄，随机选取一半孕鼠，剖腹观察胚胎吸收率。剩余母鼠继续饲养，观察分娩情况及产后仔鼠生长发育情况。结果：(1)持续光照条件下的日节律紊乱，可以导致大鼠不良妊娠结局，胚胎吸收率上升，活产仔鼠数量下降；(2)持续光照条件下的日节律紊乱，可以抑制褪黑素分泌，降低孕酮水平；(3)持续光照条件下的日节律紊乱，可以干扰大鼠的分娩节律，导致分娩时程延长，但对妊娠天数没有产生影响；(4)孕期大鼠暴露于持续光照条件下的日节律紊乱状态，可以影响仔鼠的宫内发育及子代早期生长发育。以上结果表明，持续光照导致的日节律紊乱，对于妊娠大鼠及新生大鼠，均会产生不良影响。为临床指导孕期女性生物节律调节以及褪黑素的应用，提供理论依据。

关键词： 日节律紊乱; 持续光照; 褪黑素; 妊娠大鼠; 子代

分类号：R711

[The influence of interfered circadian rhythm on pregnancy and neonatal rats.] [Article in Chinese]

CHEN Wen-Jun, SHENG Wen-Jie, GUO Yin-Hua, TAN Yong^*

The First Clinical Medical School of Nanjing University of Chinese Medicine，Nanjing 210023，China

Abstract

The aim of this study was to observe the influence of interfered circadian rhythm on pregnancy of rats and growth of neonatal rats, and to explore the relationship between the interfered circadian rhythm and the changes of melatonin and progesterone. Continuous light was used to inhibit melatonin secretion and therefore the interfered circadian rhythm animal model was obtained. The influence of interfered circadian rhythm on delivery of pregnant rats was observed. Serum was collected from rats during different stages of pregnancy to measure the concentrations of melatonin and progesterone. In order to observe the embryo resorption rate, half of pregnant rats were randomly selected to undergo a laparotomy, and the remainder was used to observe delivery and assess the growth of neonatal rats after delivery. The results showed that the interfered circadian rhythm induced adverse effects on pregnancy outcomes, including an increase of embryo resorption rate and a decrease in the number of live births; inhibited the secretion of melatonin along with decreased serum progesterone level; prolonged the stage of labor, but not the duration of pregnancy; and disturbed the fetal intrauterine growth and the growth of neonatal rats. The results suggest that interfered circadian rhythm condition made by continuous light could make adverse effects on both pregnant rats and neonatal rats. The results of our study may provide a way to modulate pregnant women’s circadian rhythm and a possibility of application of melatonin on pregnant women.

Key words: interfered circadian rhythm; continuous light; melatonin; pregnant rats; neonatal rats

收稿日期：2015-03-05　　录用日期：2015-04-28

通讯作者：谈勇　　E-mail: xijun1025@163.com

引用本文：

陈文俊, 盛文洁, 郭银华, 谈勇. 日节律紊乱对大鼠妊娠及子代的影响[J]. 生理学报 2015; 67 (5): 521-526.

CHEN Wen-Jun, SHENG Wen-Jie, GUO Yin-Hua, TAN Yong. [The influence of interfered circadian rhythm on pregnancy and neonatal rats.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (5): 521-526 (in Chinese with English abstract).