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多巴胺D1、D2受体在大鼠前扣带皮层钙结合蛋白阳性中间神经元的分布

徐蕾, 张雪寒^*

复旦大学神经生物学研究所，脑科学研究院，医学神经生物学国家重点实验室，上海 200032

摘要

大量研究表明多巴胺在行为决策、注意力调控和学习记忆等与前扣带皮层(anterior cingulate cortex, ACC)密切相关的认知功能中发挥重要作用。但是，多巴胺受体在ACC神经元上的分布还不清楚，尤其是在中间神经元上的分布。本研究旨在采用免疫组织化学和激光共聚焦扫描显微镜技术，研究多巴胺D1和D2受体在大鼠ACC一类主要类型的中间神经元，即表达钙结合蛋白的中间神经元上的分布。结果显示，D1和D2受体在ACC的小清蛋白(parvalbumin, PV)，钙结合蛋白(calbindin D-28k, CB)及钙视网膜蛋白(calretinin, CR)阳性的中间神经元均有分布。其中，D1和D2受体在PV阳性中间神经元上表达较多，在CR阳性中间神经元上表达最少；D1和D2受体在ACC深层PV神经元上的表达比例显著高于浅层。此外，CR阳性中间神经元上D2受体分布比D1受体多。D1和D2受体的这种区域和中间神经元类型特异性的表达，为了解多巴胺对ACC功能的复杂调控提供了形态学基础。

关键词： D1-多巴胺受体; D2-多巴胺受体; 中间神经元; 前扣带皮层; 大鼠

分类号：Q42

Distribution of D1 and D2-dopamine receptors in calcium-binding-protein expressing interneurons in rat anterior cingulate cortex

XU Lei, ZHANG Xue-Han^*

Institute of Neurobiology and State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Fudan University, Shanghai 200032, China

Abstract

Dopamine plays an important role in cognitive functions including decision making, attention, learning and memory in the anterior cingulate cortex (ACC). However, little is known about dopamine receptors (DAR) expression patterns in ACC neurons, especially GABAergic interneurons. The aim of the present study was to investigate the expression of the most abundant DAR subtypes, D1 receptors (D1Rs) and D2 receptors (D2Rs), in major types of GABAergic interneurons in rat ACC, including parvalbumin (PV)-, calretinin (CR)-, and calbindin D-28k (CB)-containing interneurons. Double immunofluorescence staining and confocal scanning were used to detect protein expression in rat brain sections. The results showed a high proportion of PV-containing interneurons express D1Rs and D2Rs, while a low proportion of CR-positive interneurons express D1Rs and D2Rs. D1R- and D2R-expressing PV interneurons are more prevalently distributed in deep layers than superficial layers of ACC. Moreover, we found the proportion of D2Rs expressed in CR cells is much greater than that of D1Rs. These regional and interneuron type-specific differences of D1Rs and D2Rs indicate functionally distinct roles for dopamine in modulating ACC activities via stimulating D1Rs and D2Rs.

Key words: dopamine D1 receptor; dopamine D2 receptor; interneuron; anterior cingulate cortex; rat

收稿日期：2014-10-21　　录用日期：2015-01-28

通讯作者：张雪寒　　E-mail: xuehanzhang@fudan.edu.cn

引用本文：

徐蕾, 张雪寒. 多巴胺D1、D2受体在大鼠前扣带皮层钙结合蛋白阳性中间神经元的分布[J]. 生理学报 2015; 67 (2): 163-172.

XU Lei, ZHANG Xue-Han. Distribution of D1 and D2-dopamine receptors in calcium-binding-protein expressing interneurons in rat anterior cingulate cortex. Acta Physiol Sin 2015; 67 (2): 163-172 (in Chinese with English abstract).