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大黄素增加BKCa通道β1亚基表达介导自发性高血压大鼠脑基底动脉舒张

张传林, 丛丽娜, 王蕊, 王洋, 马克涛, 赵磊, 司军强, 李 丽

石河子大学医学院生理教研室/新疆地方与民族高发病教育部重点实验室，石河子832000

摘要

本文旨在研究大黄素干预后自发性高血压大鼠(spontaneously hypertensive rat, SHR)脑基底动脉平滑肌细胞BKCa通道电生理特性和表达的变化。在使用大黄素干预处理前后，用尾动脉测压仪观察血压变化，用全细胞膜片钳记录技术观察SHR脑基底动脉平滑肌细胞BKCa通道电流的变化，用免疫组织化学染色和Western Blot观察平滑肌细胞BKCa通道表达变化。结果显示，大黄素干预后，SHR血压由(223 ± 16) mmHg显著降低至(127 ± 12) mmHg (P < 0.01)，与Wistar大鼠相比没有统计学差异。大黄素显著增加SHR平滑肌细胞外向电流(P < 0.05)，该增强作用可被BKCa通道特异性阻断剂IbTX所阻断。大黄素干预后SHR基底动脉平滑肌BKCa通道β1亚基表达明显上调(P < 0.01)。以上结果提示，大黄素可能通过增加BKCa通道β1亚基表达，增强BKCa通道介导的外向电流，从而发挥舒张SHR脑基底动脉的作用。

关键词： 脑基底动脉; 平滑肌细胞; 大黄素; 大电导钙激活钾通道; 钙离子

分类号：R1

[Emodin-induced increase in expression of β1 subunits of BKCa channel mediates relaxation of cerebral basilar artery in spontaneously hypertensive rats.] [Article in Chinese]

ZHANG Chuan-Lin, CONG Li-Na, WANG Rui, WANG Yang, MA Ke-Tao, ZHAO Lei, SI Jun-Qiang, LI Li

Electrophysiological Laboratory, Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University Medical College, Shihezi 832002, China

Abstract

The purposes of this study were to investigate the effect of emodin on expression of BK_Ca channel β₁ subunit in basilar artery smooth muscle cells (BASMCs) and electrophysiological characteristics of vascular smooth muscle cells in spontaneously hypertensive rats (SHR). Tail artery pressure measurement instrument was used to measure the change of SHR systolic blood pressure before and after emodin intervention. Single vascular smooth muscle cell was electrically recorded by whole-cell patch-clamp technique. Immunohistochemistry and Western blotting were used to study the distribution and expression of the BK_Ca channel β₁ subunit. The results showed that emodin decreased blood pressure of SHR from (223 ± 16) mmHg to (127 ± 12) mmHg (P < 0.01). There was no difference of blood pressure between emodin-treated SHR and Wistar rats. Emodin significantly increased outward currents of smooth muscle cells in SHR (P < 0.05), and this effect could be reversed by specific inhibitor of BK_Ca channel, IbTX. Emodin also up-regulated BK_Ca channel β₁ subunit expression in BASMCs. These results suggest that emodin relaxes cerebral basilar artery by enhancing BK_Ca current via increasing β₁ subunit expression in BASMCs.

Key words: basilar artery; smooth muscle cell; BKCa channel; emodin; Ca2+

收稿日期：2013-09-12　　录用日期：2013-12-13

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引用本文：

张传林, 丛丽娜, 王蕊, 王洋, 马克涛, 赵磊, 司军强, 李 丽. 大黄素增加BKCa通道β1亚基表达介导自发性高血压大鼠脑基底动脉舒张[J]. 生理学报 2014; 66 (3): 289-294.

ZHANG Chuan-Lin, CONG Li-Na, WANG Rui, WANG Yang, MA Ke-Tao, ZHAO Lei, SI Jun-Qiang, LI Li. [Emodin-induced increase in expression of β1 subunits of BKCa channel mediates relaxation of cerebral basilar artery in spontaneously hypertensive rats.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (3): 289-294 (in Chinese with English abstract).