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钙激活性氯离子通道在低氧性肺血管双相收缩反应中的作用

应磊, 姚海霞, 黄林静, 马迎春, 何金波, 陈丹, 陈海娥, 汪洋, 王万铁

温州医科大学1基础医学院病理生理学教研室；缺血-再灌注损伤研究所，温州 325035；上海市第六人民医院麻醉科，上海2 00233

摘要

本文旨在探讨钙激活性氯离子通道(ClCa)在二级肺动脉血管低氧性肺血管收缩(hypoxic pulmonary vasoconstriction, HPV)中的作用。分离Sprague-Dawley (SD)雄性大鼠二级肺动脉血管，采用离体血管灌流法记录血管环张力变化。结果显示，常氧条件下，ClCa抑制剂尼氟灭酸(10和50 μmol/L)和IAA-94 (10 μmol/L)使去甲肾上腺素收缩的二级肺动脉环血管产生明显的舒张反应(P < 0.01)，但对KCl收缩的二级肺动脉环血管并无影响。低氧条件下1 h内，去甲肾上腺素预收缩的二级肺动脉血管环出现双相性HPV反应，KCl预收缩的血管环无双相性收缩反应。尼氟灭酸和IAA-9明显减弱II期收缩反应(均P < 0.01)，对I期舒张反应也有显著减弱作用(均P < 0.01)，但对I期收缩几乎无影响。以上结果提示，ClCa是二级肺动脉血管双相性收缩反应II期收缩形成的一个重要因素，而在I期收缩中无明显作用。

关键词： 钙激活性氯离子通道; 肺动脉; 低氧性肺血管收缩

分类号：R329

[Calcium-activated chloride channels are involved in two-phase hypoxic pulmonary vasoconstriction in rat pulmonary arteries.] [Article in Chinese]

YING Lei, YAO Hai-Xia, HUANG Lin-Jing, MA Ying-Chun, HE Jin-Bo, CHEN Dan, CHEN Hai-E, WANG Yang, WANG Wan-Tie

Department of Pathophysiology； Institute of Ischemia-reperfusion Injury,Wenzhou Medical University, Wenzhou 325035, China； Department of Anesthesiology, The Sixth People’s Hospital of Shanghai , Shanghai 200233 , China

Abstract

The aim of the present study was to investigate the roles of calcium-activated chloride channels (Cl_Ca) in the two-phase hypoxic pulmonary vasoconstriction (HPV). The second pulmonary artery branches were dissected from male Sprague-Dawley rats, and the changes in vascular tone were measured by using routine blood vascular perfusion in vitro. The result showed that, under normoxic conditions, Cl_Ca inhibitors (NFA and IAA-94) significantly relaxed second pulmonary artery contracted by norepinephrine (P < 0.01), but merely had effects on KCl-induced second pulmonary artery contractions. A biphasic contraction response was induced in second pulmonary artery ring pre-contracted with norepinephrine exposed to hypoxic conditions for at least one hour, but no biphasic contraction was observed in pulmonary rings pre-contracted with KCl. NFA and IAA-94 significantly attenuated phase II sustained hypoxic contraction (P < 0.01), and also attenuated phase I vasodilation, but had little effect on phase I contraction. These results suggest that Cl_Ca is an important component forming phase II contraction in secondary pulmonary artery, but not involved in phase I contraction.

Key words: Calcium-activated chloride channels; pulmonary artery; Hypoxic pulmonary vasoconstriction

收稿日期：2013-08-14　　录用日期：2014-01-07

通讯作者：王万铁　　E-mail: wzwwt@tom.com

引用本文：

应磊, 姚海霞, 黄林静, 马迎春, 何金波, 陈丹, 陈海娥, 汪洋, 王万铁. 钙激活性氯离子通道在低氧性肺血管双相收缩反应中的作用[J]. 生理学报 2014; 66 (2): 203-209.

YING Lei, YAO Hai-Xia, HUANG Lin-Jing, MA Ying-Chun, HE Jin-Bo, CHEN Dan, CHEN Hai-E, WANG Yang, WANG Wan-Tie. [Calcium-activated chloride channels are involved in two-phase hypoxic pulmonary vasoconstriction in rat pulmonary arteries.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (2): 203-209 (in Chinese with English abstract).