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局部应用奥曲肽对大鼠背部皮神经跨节段交互兴奋的抑制效应

王军, 曹东元, 郭媛, 马少杰, 赵晏

河南大学医学院解剖学教研室，开封 475001；西安交通大学生理学与病理生理学系，西安 710061；美国马里兰大学神经和疼痛学系，马里兰 21201，美国

摘要

本研究在大鼠背部皮下感受野微量注射生长抑素(somatostatin, SST)类似物奥曲肽(octreotide, OCT)，观察OCT对大鼠背部皮神经跨节段交互兴奋的作用及可能机制。选取大鼠T9~T13脊神经背侧皮支，游离后进行刺激和记录。逆行电刺激相邻节段神经干，记录单纤维的机械阈值和放电频率。用感受野皮下注射SST类似物OCT或SST受体拮抗剂环生长抑素(cyclo-somatostatin, c-SOM)进行干预，观察交互兴奋特征改变。结果显示：感受野皮下注射OCT可以抑制皮神经交互兴奋引起的放电频率增加和机械阈值降低；c-SOM可以翻转OCT的抑制效应；单纯应用c-SOM可以显著增强交互兴奋现象。以上结果提示，局部应用SST类似物OCT可通过SST受体对大鼠皮神经跨节段的交互兴奋现象产生抑制效应。

关键词： 生长抑素受体; 脊神经; 交互兴奋

分类号：Q429

[Inhibitory effect of octreotide on the cross excitation of adjacent segment of spinal nerve in rat] [Article in Chinese]

Wang Jun, CAO DONG-YUAN, GUO Yuan, MA Shao-Jie, ZHAO Yan

Department of Anatomy, Henan University College of Medicine, Kaifeng 475004, China； Department of Physiology, Xi’an Jiaotong University School of Medicine, Xi’an 710061, China； Department of Neural and Pain Sciences University of Maryland School of Dentistry, Baltimore, Maryland 21201, USA

Abstract

This study was to observe the effect and possible mechanism of somatostatin analogue octreotide (OCT) on cross excitation of adjacent segment of spinal nerve in rat. Cutaneous branches of T9-T13 spinal dorsal rami were chosen and dissected free for the following recording and stimulation. Only single unit fiber was used for recording, and the adjacent segment of nerve stem was used for antidromic electrical stimulation. To investigate the change of discharge rate and mechanical threshold, OCT and (or) somatostatin receptor antagonist cyclo-somatostatin (c-SOM) were applied to the receptive field following the antidromic electrical stimulation. The result showed that injection of OCT inhibited the increase of discharge rate and the decrease of mechanical threshold induced by the electrical stimulation (cross excitation); c-SOM reversed the effects of OCT. Application of c-SOM alone enhanced the cross excitation effects. The results suggest local application of somatostatin analogue OCT can inhibit the cross excitation between the two segments of spinal nerve by somatostatin receptor.

Key words: Somatostatin receptor; spinal nerve; cross excitation

收稿日期：2013-05-28　　录用日期：2013-09-04

通讯作者：曹东元,赵晏　　E-mail: zhaoy502@mail.xjtu.edu.cn, Dongyuan_cao@hotmail.com

引用本文：

王军, 曹东元, 郭媛, 马少杰, 赵晏. 局部应用奥曲肽对大鼠背部皮神经跨节段交互兴奋的抑制效应[J]. 生理学报 2013; 65 (6): 593-599.

Wang Jun, CAO DONG-YUAN, GUO Yuan, MA Shao-Jie, ZHAO Yan. [Inhibitory effect of octreotide on the cross excitation of adjacent segment of spinal nerve in rat] [Article in Chinese]. Acta Physiol Sin 2013; 65 (6): 593-599 (in Chinese with English abstract).