新型雌激素受体ER-α36在新生与成年大鼠海马和皮层中表达的比较
刘洋, 方晨, 邹萍, 马依妮, 韩丹女, 嵇志红, 梁小峰, 关心, 黄亮, 冯田, 王一婷, 刘晶, 邹伟
辽宁师范大学生命科学学院,大连 116081;辽宁师范大学发展和教育心理学研究中心,大连 116029;大连医科大学附属第一医院中英再生医学中心,大连 116011;大连大学师范学院,大连 116622;大连大学医学院,大连 116622;复旦大学生命科学学院,上海 200433
摘要
ER-α36是一种新型雌激素受体亚型,广泛分布于乳腺、子宫、消化道、呼吸道等组织和细胞中。本文旨在研究ER-α36在中枢神经系统中的表达和分布情况。采用免疫细胞/组织化学方法和蛋白质免疫印迹杂交比较ER-α36在新生(1日龄)与成年(12周龄) Sprague-Dawley (SD)大鼠海马及皮层区的表达和分布情况。研究显示,ER-α36在成年大鼠海马及皮层区均有表达,主要分布于锥体神经元。ER-α36在新生大鼠海马和皮层神经元中均定位于细胞膜。新生大鼠皮层神经元ER-α36蛋白表达高于海马,而成年大鼠皮层神经元ER-α36蛋白表达低于海马。与新生大鼠相比较,成年大鼠海马和皮层神经元ER-α36表达均显著提高。以上结果提示,ER-α36参与介导神经元膜雌激素信号通路的调节,且在大鼠出生后海马及皮层发育中发挥着重要作用。因此,ER-α36可能为学习记忆、神经系统退行性疾病等的防治提供潜在的药物靶点。
关键词: ER-α36; 中枢神经系统; 出生后脑发育; 原代培养神经元
分类号:Q257
[Diverse expression of ER-α36, a novel variant of ER-α, in hippocampus and cortex of neonatal and adult rats.] [Article in Chinese]
Liu Yang, FANG Chen, ZOU Ping, MA Yi-Ni, HAN Dan-Nv, JI Zhi-Hong, LIANG Xiao-Feng, GUAN Xin, HUANG Liang, FENG Tian, WANG Yi-Ting, LIU Jing, ZOU Wei
College of Life Science, Liaoning Normal University, Dalian 116081, China; The Research Center of Developmental and Educational Psychology, Liaoning Normal University, Dalian 116029, China; Regenerative Medicine Centre, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China; Teacher's College, Dalian University, Dalian 116622, China; Medical College, Dalian University, Dalian 116622, China; School of Life Science, Fudan University, Shanghai 200433, China. E-mail: weizou60@126.com; liujing.dlrmc@hotmail.com
Abstract
ER-α36, a novel variant of ER-α, is expressed in breast, uterus, digestive tract, respiratory tract etc. The aim of the present study was to investigate the distribution and expression of ER-α36 in the central nervous system (CNS). Here, we comparatively analyzed the expression pattern of ER-α36 in the hippocampus and cortex of neonatal (1-day-old) and adult (12-week-old) Sprague-Dawley (SD) rats by using immunohistochemistry/immunocytochemistry analysis and Western blot. The results showed that ER-α36 was expressed both in hippocampus and cortex of adult rats, but mainly distributed in pyramidal neurons. ER-α36 was mainly located on the cytomembrane of hippocampal and cortical neurons from neonatal rats. Compared with the cortical neurons, the hippocampal neurons showed lower ER-α36 protein expression in the neonatal rats, but exhibited higher level of ER-α36 in the adult rats. Furthermore, the adult rats showed higher levels of ER-α36 expression in both hippocampus and cortex compared with the neonatal rats. These results suggest that ER-α36 might be involved in the regulation of membrane-initiated estrogen signaling throughout the postnatal development of diverse brain regions, and thus will be a potential target for the treatment of degenerative diseases in nervous system.
Key words: ER-α36; central nervous system; growth and development; primary culture
收稿日期:2012-09-04 录用日期:2013-01-06
通讯作者:邹伟 E-mail: weizou60@126.com
引用本文:
刘洋, 方晨, 邹萍, 马依妮, 韩丹女, 嵇志红, 梁小峰, 关心, 黄亮, 冯田, 王一婷, 刘晶, 邹伟. 新型雌激素受体ER-α36在新生与成年大鼠海马和皮层中表达的比较[J]. 生理学报 2013; 65 (3): 263-268.
Liu Yang, FANG Chen, ZOU Ping, MA Yi-Ni, HAN Dan-Nv, JI Zhi-Hong, LIANG Xiao-Feng, GUAN Xin, HUANG Liang, FENG Tian, WANG Yi-Ting, LIU Jing, ZOU Wei. [Diverse expression of ER-α36, a novel variant of ER-α, in hippocampus and cortex of neonatal and adult rats.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (3): 263-268 (in Chinese with English abstract).