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新型雌激素受体ER-α36在新生与成年大鼠海马和皮层中表达的比较

刘洋, 方晨, 邹萍, 马依妮, 韩丹女, 嵇志红, 梁小峰, 关心, 黄亮, 冯田, 王一婷, 刘晶, 邹伟

辽宁师范大学生命科学学院，大连 116081；辽宁师范大学发展和教育心理学研究中心，大连 116029；大连医科大学附属第一医院中英再生医学中心，大连 116011；大连大学师范学院，大连 116622；大连大学医学院，大连 116622；复旦大学生命科学学院，上海 200433

摘要

ER-α36是一种新型雌激素受体亚型，广泛分布于乳腺、子宫、消化道、呼吸道等组织和细胞中。本文旨在研究ER-α36在中枢神经系统中的表达和分布情况。采用免疫细胞/组织化学方法和蛋白质免疫印迹杂交比较ER-α36在新生(1日龄)与成年(12周龄) Sprague-Dawley (SD)大鼠海马及皮层区的表达和分布情况。研究显示，ER-α36在成年大鼠海马及皮层区均有表达，主要分布于锥体神经元。ER-α36在新生大鼠海马和皮层神经元中均定位于细胞膜。新生大鼠皮层神经元ER-α36蛋白表达高于海马，而成年大鼠皮层神经元ER-α36蛋白表达低于海马。与新生大鼠相比较，成年大鼠海马和皮层神经元ER-α36表达均显著提高。以上结果提示，ER-α36参与介导神经元膜雌激素信号通路的调节，且在大鼠出生后海马及皮层发育中发挥着重要作用。因此，ER-α36可能为学习记忆、神经系统退行性疾病等的防治提供潜在的药物靶点。

关键词： ER-α36; 中枢神经系统; 出生后脑发育; 原代培养神经元

分类号：Q257

[Diverse expression of ER-α36, a novel variant of ER-α, in hippocampus and cortex of neonatal and adult rats.] [Article in Chinese]

Liu Yang, FANG Chen, ZOU Ping, MA Yi-Ni, HAN Dan-Nv, JI Zhi-Hong, LIANG Xiao-Feng, GUAN Xin, HUANG Liang, FENG Tian, WANG Yi-Ting, LIU Jing, ZOU Wei

College of Life Science, Liaoning Normal University, Dalian 116081, China； The Research Center of Developmental and Educational Psychology, Liaoning Normal University, Dalian 116029, China； Regenerative Medicine Centre, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China； Teacher's College, Dalian University, Dalian 116622, China； Medical College, Dalian University, Dalian 116622, China； School of Life Science, Fudan University, Shanghai 200433, China. E-mail: weizou60@126.com； liujing.dlrmc@hotmail.com

Abstract

ER-α36, a novel variant of ER-α, is expressed in breast, uterus, digestive tract, respiratory tract etc. The aim of the present study was to investigate the distribution and expression of ER-α36 in the central nervous system (CNS). Here, we comparatively analyzed the expression pattern of ER-α36 in the hippocampus and cortex of neonatal (1-day-old) and adult (12-week-old) Sprague-Dawley (SD) rats by using immunohistochemistry/immunocytochemistry analysis and Western blot. The results showed that ER-α36 was expressed both in hippocampus and cortex of adult rats, but mainly distributed in pyramidal neurons. ER-α36 was mainly located on the cytomembrane of hippocampal and cortical neurons from neonatal rats. Compared with the cortical neurons, the hippocampal neurons showed lower ER-α36 protein expression in the neonatal rats, but exhibited higher level of ER-α36 in the adult rats. Furthermore, the adult rats showed higher levels of ER-α36 expression in both hippocampus and cortex compared with the neonatal rats. These results suggest that ER-α36 might be involved in the regulation of membrane-initiated estrogen signaling throughout the postnatal development of diverse brain regions, and thus will be a potential target for the treatment of degenerative diseases in nervous system.

Key words: ER-α36; central nervous system; growth and development; primary culture

收稿日期：2012-09-04　　录用日期：2013-01-06

通讯作者：邹伟　　E-mail: weizou60@126.com

引用本文：

刘洋, 方晨, 邹萍, 马依妮, 韩丹女, 嵇志红, 梁小峰, 关心, 黄亮, 冯田, 王一婷, 刘晶, 邹伟. 新型雌激素受体ER-α36在新生与成年大鼠海马和皮层中表达的比较[J]. 生理学报 2013; 65 (3): 263-268.

Liu Yang, FANG Chen, ZOU Ping, MA Yi-Ni, HAN Dan-Nv, JI Zhi-Hong, LIANG Xiao-Feng, GUAN Xin, HUANG Liang, FENG Tian, WANG Yi-Ting, LIU Jing, ZOU Wei. [Diverse expression of ER-α36, a novel variant of ER-α, in hippocampus and cortex of neonatal and adult rats.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (3): 263-268 (in Chinese with English abstract).