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白藜芦醇甙增强大鼠心室肌细胞内向整流钾通道电流

魏燕, 周京京, 杨晶, 张娇, 张利萍, 张翼

河北医科大学生理学教研室，石家庄 050017；泸州医学院心血管病研究所电生理研究室，泸州 646000；河北省医学生物技术重点实验室，石家庄 050017

摘要

本研究旨在应用全细胞膜片钳技术观察白藜芦醇甙(polydatin)对大鼠心室肌细胞瞬时外向钾通道电流(Ito)、稳态外向钾通道电流(Iss)和内向整流钾通道电流(IK1)的影响。结果显示：(1)白藜芦醇甙(10 µmol/L以上浓度)可通过非浓度依赖性方式增加心室肌细胞IK1。(2)白藜芦醇甙对心室肌细胞Ito无影响，对Ito的激活、失活和失活后的恢复动力学亦无影响。(3)白藜芦醇甙对心室肌细胞Iss无明显影响。以上结果提示，白藜芦醇甙对大鼠心室肌细胞IK1具有激活作用，可增加IK1，但对Ito和Iss无明显影响；白藜芦醇甙对IK1的作用可能是其抗心律失常作用的离子机制之一。

关键词： 白藜芦醇甙; 瞬时外向钾通道; 稳态外向钾通道; 内向整流钾通道; 心肌细胞; 大鼠

Enhancement of polydatin on inward rectifier potassium channel current in rat ventricular myocytes

WEI Yan, ZHOU Jing-Jing, Yang Jing, ZHANG Jiao, ZHANG Li-Ping, Zhang Yi

Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China； Department of Electrophysiology, Institute of Cardiovascular Research, Luzhou Medical College, Luzhou 646000, China； Hebei Key Laboratory of Medical Biotechnology, Shijiazhuang 050017, China

Abstract

The aim of this study was to investigate the effect of polydatin on transient outward potassium channel current (I_to), steady-state outward potassium channel current (I_ss) and inward rectifier potassium channel current (I_K1) in ventricular myocytes of rat using the whole-cell patch clamp technique. The results showed: (1) Polydatin (above 10 µmol/L) increased I_K1 of ventricular myocytes in a non-concentration dependent manner. (2) Polydatin neither had any effect on I_to peak current of ventricular myocytes, nor changed activation, inactivation and recovery kinetics of I_to. (3) Polydatin had no effect on I_ss of ventricular myocytes. These results suggest that polydatin enhances I_K1 channel activity, but has no effect on I_to and I_ss channels in rat ventricular myocytes, which might be one of the ionic mechanisms for antiarrhythmic effect of polydatin

Key words: polydatin; transient outward potassium channel; steady-state outward potassium channel; inward rectifier potassium channel; cardiomyocytes; rat

收稿日期：2012-09-13　　录用日期：2013-01-08

通讯作者：张翼　　E-mail: zhyhenry@hotmail.com

引用本文：

魏燕, 周京京, 杨晶, 张娇, 张利萍, 张翼. 白藜芦醇甙增强大鼠心室肌细胞内向整流钾通道电流[J]. 生理学报 2013; 65 (3): 285-292.

WEI Yan, ZHOU Jing-Jing, Yang Jing, ZHANG Jiao, ZHANG Li-Ping, Zhang Yi. Enhancement of polydatin on inward rectifier potassium channel current in rat ventricular myocytes. Acta Physiol Sin 2013; 65 (3): 285-292 (in Chinese with English abstract).