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骨髓内皮细胞来源的可溶性因子调节造血系内皮系及胚胎干细胞的分化和增殖

王绮如, 阎琦

中南大学湘雅医学院生理学系，长沙 410078；华盛顿大学生物结构与眼科学系，Benaroya 研究所，西雅图，华盛顿州 98195，美国

摘要

本研究室建立了一支骨髓内皮细胞(bone marrow endothelial cell, BMEC)株。本文综述了这一内皮细胞株细胞的条件培养液(bone marrow endothelial cell-conditioned medium, BMEC-CM)对造血系、内皮系及胚胎干细胞的分化增殖的作用。 (1) BMEC-CM促进造血系细胞的分化和增殖；(2) BMEC-CM促进内皮系细胞的分化和增殖；(3) BMEC-CM诱导造血干/祖细胞向内皮系细胞分化；(4) BMEC-CM诱导胚胎干细胞分化为造血细胞和内皮细胞。以上研究成果提示，骨髓内皮细胞分泌可溶性因子支持造血系及内皮系细胞的分化与增殖，促进造血系细胞向内皮系细胞的横向分化，诱导胚胎干细胞分化为造血细胞和内皮细胞。本文进一步阐述了造血系与内皮系之间的密切关系，对缺血性疾病及肿瘤疾病提供有效治疗策略。

关键词： 分化与增殖; 内皮系; 造血系; 胚胎干细胞

Soluble factors from bone marrow endothelial cells regulate differentiation and proliferation of hematopoietic and endothelial lineages and embryonic stem cells

WANG Qi-Ru, YAN Qi

Department of Physiology, Xiangya Medical School of Central South University, Changsha 410078, China； Benaroya Research Institute, Departments of Biological Structure and Ophthalmology, University of Washington, Seattle, WA 98195, USA

Abstract

We have established a bone marrow endothelial cell line. This review focuses on the elucidation and analysis of the effects of this bone marrow endothelial cell-conditioned medium (BMEC-CM) on the differentiation and proliferation of hematopoietic and endothelial progenitors as well as embryonic stem cells (ESCs). We will review that (1) BMEC-CM promotes proliferation and differentiation of hematopoietic lineage; (2) BMEC-CM promotes proliferation and differentiation of endothelial lineage; (3) BMEC-CM induces differentiation of hematopoietic stem cells/progenitors into endothelial progenitors; and (4) BMEC-CM induces differentiation of ESCs into hematopoietic cells and endothelial cells. We conclude that the soluble factors secreted by BMECs are able to support the proliferation and differentiation of hematopoietic and endothelium lineages. Moreover, these soluble factors induce hematopoietic cells to differentiate to endothelial cells, and induce ESCs to differentiate towards both endothelial cells and hematopoietic cells. Therefore, this work provides evidence that a close relationship involved in the development of hematopoietic and endothelial lineage. This disclosure will be beneficial for therapy strategy in the treatment of ischemic and tumor diseases, and improve our understanding of the relationship between hematopoietic and endothelial lineages.

Key words: differentiation and proliferation; endothelial lineage; hematopoietic lineage; embryonic stem cells

收稿日期：2013-01-31　　录用日期：2013-06-28

通讯作者：王绮如　　E-mail: qirwang@yahoo.com

引用本文：

王绮如, 阎琦. 骨髓内皮细胞来源的可溶性因子调节造血系内皮系及胚胎干细胞的分化和增殖[J]. 生理学报 2013; 65 (4): 433-444.

WANG Qi-Ru, YAN Qi. Soluble factors from bone marrow endothelial cells regulate differentiation and proliferation of hematopoietic and endothelial lineages and embryonic stem cells. Acta Physiol Sin 2013; 65 (4): 433-444 (in Chinese with English abstract).