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类固醇激素合成酶在大鼠多囊卵巢综合征模型中的表达

林伟, 宋张娟, 孙玮明, 董磊, 金可可, 王万铁, 汪洋

温州医学院病理生理教研室；附属第一医院病理科，温州 325035

摘要

本文旨在探讨类固醇激素合成酶在多囊卵巢综合征(polycystic ovary syndrome, PCOS)中的表达变化。将Sprague-Dawley (SD)大鼠随机分为正常对照组(NC组)和多囊卵巢综合征组(PCOS组)，PCOS组大鼠注射脱氢表雄酮(dehydroepiandrosterone, DHEA)以建立PCOS模型，用放射免疫法测血清激素(孕酮和雌二醇)水平，酶联免疫法检测血清睾酮水平。取各组卵巢组织，用免疫组化法对3β-羟基固醇脱氢酶(3β-hydroxy steroid dehydrogenase, 3β-HSD)、17β-羟基固醇脱氢酶(17β-hydroxy steroid dehydrogenase, 17β-HSD)和细胞色素P450芳香化酶(cytochrome P450 aromatase, P450arom)进行细胞定位分析，RT-PCR和Western blot分别检测这三种酶的mRNA和蛋白表达变化。结果显示，与NC组相比，PCOS组血清雌二醇和睾酮水平显著升高，孕酮无显著变化。与NC组相比，PCOS组3β-HSD和17β-HSD mRNA和蛋白表达均显著升高，P450arom的mRNA和蛋白表达则明显降低。以上结果提示，3β-HSD和17β-HSD共同参与了PCOS大鼠模型卵巢的激素调节，P450arom并未直接参与雌二醇的调节。

关键词： 多囊卵巢综合征; 大鼠; 3β-羟基固醇脱氢酶; 17β-羟基固醇脱氢酶; 细胞色素 P450 芳香化酶

分类号：R711.75

[Expression of steroidogenic enzymes in the rat model of polycystic ovary syndrome.] [Article in Chinese]

LIN Wei, SONG Zhang-Juan, SUN Wei-Ming, DONG Lei, JIN Ke-Ke, WANG Wan-Tie, WANG Yang

Department of Pathophysiology； Department of Pathology of the First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325035, China

Abstract

The aim of the present study was to investigate the expression changes of three steroidogenic enzymes in the polycystic ovary syndrome (PCOS). Thirty Sprague-Dawley (SD) rats were randomly divided into normal control (NC) group and PCOS group. PCOS rat model was established by DHEA injection. The serum levels of progesterone, estrogen and testosterone were measured by immunoradioassay or enzyme immunoassay. The cellular distributions of 3β-hydroxy steroid dehydrogenase (3β-HSD), 17β-hydroxy steroid dehydrogenase (17β-HSD) and cytochrome P450 aromatase (P450arom) in ovaries were detected by immunohistochemistry. The expression levels of 3β-HSD, 17β-HSD and P450arom were detected by RT-PCR and Western blot. The results showed that the serum levels of estrogen and testosterone of PCOS group were significantly higher than those of the NC group. There was no significant difference of serum progesterone level between the PCOS and NC groups. Compared with the NC group, the PCOS group showed increased mRNA and protein expressions of both 3β-HSD and 17β-HSD, as well as reduced P450arom mRNA and protein expressions. These results suggest that 3β-HSD and 17β-HSD, but not P450arom, may participate in the ovarian hormonal regulation in the present rat model of PCOS.

Key words: polycystic ovary syndrome; rat; 3β-hydroxy steroid dehydrogenase; 17β-hydroxy steroid dehydrogenase; cytochrome P450 aromatase

收稿日期：2012-07-21　　录用日期：2012-09-26

通讯作者：汪洋　　E-mail: wangydh995@163.com

引用本文：

林伟, 宋张娟, 孙玮明, 董磊, 金可可, 王万铁, 汪洋. 类固醇激素合成酶在大鼠多囊卵巢综合征模型中的表达[J]. 生理学报 2013; 65 (2): 171-177.

LIN Wei, SONG Zhang-Juan, SUN Wei-Ming, DONG Lei, JIN Ke-Ke, WANG Wan-Tie, WANG Yang. [Expression of steroidogenic enzymes in the rat model of polycystic ovary syndrome.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (2): 171-177 (in Chinese with English abstract).