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{sl PTEN}在早孕小鼠子宫内膜的表达及其对胚泡着床的影响

陈晓玲, 马海兰, 谢怡, 杨戎, 魏莎莉

重庆医科大学生殖生物学研究室.重庆 400016

摘要

该研究旨在检测肿瘤抑制基因{sl PTEN}(phosphatase and tensin homolog deleted on chromosome ten)在早孕小鼠子宫内膜中的表达规律,探讨{sl PTEN}在小鼠胚胎着床过程中的作用。采用实时荧光定量聚合酶联反应(real--time fluorescent quantitative PCR,FQ--PCR)和免疫组织化学方法分别检测未孕及孕 1、3、4、5、7 d 小鼠子宫内膜PTEN mRNA和蛋白的表达;子宫角注射PTEN反义寡核苷酸观察胚泡着床数。FQ--PCR结果显示,妊娠小鼠子宫内膜组织{sl PTEN} mRNA的表达高于未妊娠小鼠,且随着妊娠天数的增加表达逐渐增强,到妊娠第 5 天达最高。免疫组织化学分析显示,PTEN蛋白在子宫内膜的表达规律与mRNA 结果一致。子宫角注射{sl PTEN}反义寡核苷酸后胚泡着床数明显减少。结果提示,{sl PTEN}在妊娠早期子宫内膜持续表达,可能参与了胚泡着床。

关键词： 着床; 子宫内膜; PTEN; 实时荧光定量聚合酶联反应; 免疫组织化学; 反义寡核苷酸

Expression of phosphatase and tensin homolog deleted on chromosome ten in mouse endometrium and its effect during blastocyst implantation

Chen Xiaoling, Ma Hailan, Xie Yi, Yang Rong, Wei Shali

Laboratory of the Reproductive Biology, Chongqing Medical University.Chongqing 400016

Abstract

The present study was aimed to investigate the expression of tumor suppressor gene PTEN （phosphatase and tensin homolog deleted on chromosome ten） in mouse endometrium during early pregnancy and its possible role during blastocyst implantation. Real-time fluorescent quantitative PCR （FQ-PCR） and immunohistochemical techniques were applied to detect PTEN mRNA and protein expressions in endometrium in un-pregnant and pregnant mice on days 1, 3, 4, 5, 7 of pregnancy, respectively. In addition, PTEN antisense oligonucleotide was injected into the horns of uterus in pregnant mice on day 3 of pregnancy and its effects on blastocyst implantation was detected in vivo. The higher expressions of PTEN mRNA and protein were observed in pregnant mice compared with that in un-pregnant mice, with a steady increasing from day 1 to 7 and reaching the maximal level on day 5 of pregnancy. PTEN antisense oligonucleotide decreased the number of implanted blastocysts compared with saline. The results suggest that PTEN might associate with apoptosis of luminal epithelial and decidual cells, coordinating decidualization of endometrium and invasion of trophoblastic cells. Thus, PTEN may participate in the process of blastocyst implantation in mice.

Key words: blastocyst implantation;Endometrium;PTEN;real-time fluorescent quantitative PCR;Immunohistochemistry;antisense oligonucleotide

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引用本文：

陈晓玲, 马海兰, 谢怡, 杨戎, 魏莎莉. {sl PTEN}在早孕小鼠子宫内膜的表达及其对胚泡着床的影响[J]. 生理学报 2008; 60 (1): 119-124.

Chen Xiaoling, Ma Hailan, Xie Yi, Yang Rong, Wei Shali. Expression of phosphatase and tensin homolog deleted on chromosome ten in mouse endometrium and its effect during blastocyst implantation. Acta Physiol Sin 2008; 60 (1): 119-124 (in Chinese with English abstract).