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萎缩比目鱼肌间断强直收缩疲劳后恢复速率的影响因素

李辉, 焦博, 余志斌

第四军医大学航空航天生理学教研室.陕西,西安 710032

摘要

为研究模拟失重大鼠萎缩比目鱼肌强直收缩疲劳后恢复速率的影响因素，采用尾部悬吊模拟失重大鼠模型及离体骨骼肌条灌流技术，观测其在不同收缩模式下疲劳后的恢复过程。正常大鼠离体比目鱼肌条实验显示，10s短时程（S10P）与300s长时程（L10P）强直收缩轻度疲劳[强直收缩最大张力（P_(0)）下降10％]后，在20min恢复期末，均可恢复至疲劳前P_(0)，且恢复程度不受疲劳持续时间的影响；轻度疲劳后，在灌流液中加入10#mu#mol／L钌红抑制肌浆网Ca~(2+)释放功能，恢复速率减慢，恢复程度最大仅至94％P_(0)，然后呈下降趋势，提示轻度疲劳可能仅抑制肌原纤维功能。60s短时程（S50P）与300s长时程（L50P）强直收缩中度疲劳（P_(0)下降50％）后，在20min恢复期末，收缩张力分别恢复至95％P_(0)和90％P_(0)，表明中度疲劳持续时间影响恢复的速率；相同条件中度疲劳后，在灌流液中加入5mmol／L咖啡因促进肌浆网Ca~(2+)释放功能，恢复速率明显加快，无论疲劳持续时间长短，5min便可完全恢复，提示中度疲劳不仅抑制肌原纤维功能，还抑制肌浆网Ca~(2+)释放功能。尾部悬吊1周的大鼠比目鱼肌明显萎缩，其重量／体重之比仅为对照大鼠的60％。采用短与长持续时间的轻与中度疲劳作用后，在20min恢复期末，收缩张力分别恢复至94％P_(0)（S10P）、95％P_(0)（L10P）、92％P_(0)（S50P）、84％P_(0)（L50P），均与同步对照组有显著差异。以上结果提示：模拟失重1周大鼠萎缩的比目鱼肌，轻度与中度疲劳均可抑制肌原纤维功能与肌浆网Ca~(2+)释放功能，使恢复速率减慢。

关键词： 模拟失重; 强直收缩; 疲劳; 恢复速率

Factors modulating recovery rate after intermittent tetanic fatigue in atrophic soleus

Li Hui, Jiao Bo, Yu Zhibin

Department of Aerospace Physiology, the Fourth Military Medical University.Xi'an 710032,Shaanxi

Abstract

Fatigue occurs when the interval of intermittent tetanic contraction of skeletal muscle is shortened to a certain degree and the contractile tension declines. After fatigue, prolongation of the contraction interval can make the contractile tension recover. In atrophic soleus, the recovery rate is slower. It has been shown that a decrease in the contractile tension is caused by the inhibition of the myofibrils and sarcoplasmic reticulum Ca~(2+) release channels during fatigue. So the mechanism of the recovery of contractile tension is the recovery of the inhibited myofibrils and sarcoplasmic reticulum Ca~(2+) release channels. But how the inhibition affects the recovery course is still unclear. To specify the factors modulating the recovery rate after intermittent tetanic fatigue in soleus, and to seek the reasons for the decrease in recovery rate in atrophic soleus, we observed the recovery time course of different types of fatigue in isolated soleus muscle strips. The 10% or 50% decrease in the maximal tetanic contractile tention （P_(0)） was defined respectively as slight or moderate fatigue. After short-term （S10P, 10 s） and long-term （L10P, 300 s） slight fatigue, the tetanic contractile tension recovered to nearly 100% P_(0) at the 20th minute. In both slight fatigue groups, perfusion with 10 #mu#mol/L of ruthenium red （an inhibitor of Ca~(2+) release channels in sarcoplasmic reticulum） slowed down the recovery rate. It was suggested that slight fatigue only induced inhibition of myofibrils. After short-term （S5OP, 60 s） or long-term （L5OP, 300 s） moderate fatigue, the tetanic contractile tension at the 20th minute recovered to about 95% P_(0) in S5OP group and 90% P_(0) in L50P group, respectively. The recovery rate in L50P group was significantly lower than that in S50P group. So the recovery rate after moderate fatigue was related to the tetanic contraction duration. In both moderate fatiguegroups, perfusion with 5 mmol/L of caffeine （an opener of Ca~(2+) release channels in sarcoplasmic reticulum） resulted in nearly 100% recovery at the 5th minute. It was suggested that moderate fatigue induced inhibition of myofibrils and sarcoplasmic reticulum Ca~(2+) release channels. In 1-week tail-suspended rats, soleus muscles showed a 40% of atrophy. After slight fatigue, the tetanic contractile tension in unloaded soleus recovered to 94% P_(0) in S10P group and 95% P_(0) in L10P. After moderate fatigue, the tetanic contractile tension in unloaded soleus recovered to 92% P_(0) in S50P and 84% P0in L50P at the 20th minute. There were significant decreases in all of the fatigue groups as compared with the control groups. These results suggest that both slight and moderate fatigue inhibit the myofibrils and sarcoplasmic reticulum Ca~(2+) release channels in 1-week unloaded soleus, so the recovery rate after tetanic fatigue is slower than that in the control group.

Key words: Weightlessness simulation;tetanic contraction;Fatigue;recovery rate

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引用本文：

李辉, 焦博, 余志斌. 萎缩比目鱼肌间断强直收缩疲劳后恢复速率的影响因素[J]. 生理学报 2007; 59 (3): 369-374.

Li Hui, Jiao Bo, Yu Zhibin. Factors modulating recovery rate after intermittent tetanic fatigue in atrophic soleus. Acta Physiol Sin 2007; 59 (3): 369-374 (in Chinese with English abstract).