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间歇性低氧对肥胖小鼠瘦素及其受体表达的影响

秦岭, 宋智, 文赛兰, 井然, 李岑, 向阳, 秦晓群

中南大学湘雅医学院2002级临床医学七年制.湖南,长沙 410078；中南大学湘雅医学院生理学教研室.湖南,长沙 410078

摘要

为探讨适度低氧环境对体重的影响及其作用机制，明确瘦素在其中的作用，用高脂饮食建立小鼠肥胖模型并观察间歇性低氧的干预效果。健康昆明小鼠随机分为4组（每组20只），正常对照组：喂正常食物，不进行间歇性低氧训练；低氧组：喂正常食物，并进行间歇性低氧训练；肥胖组：喂高脂、高糖食物，但不进行间歇性低氧训练；低氧+肥胖组，喂高脂、高糖食物，并进行间歇性低氧训练。40d后，测量小鼠体重，用酶联免疫吸附法测定血清瘦素水平，免疫组织化学检测肝脏瘦素受体表达，苏丹Ⅲ染色检测肝脏脂肪细胞分布和密度。结果显示，与正常对照组相比，肥胖组小鼠平均体重和平均血清瘦素水平显著升高，肝脏分布大量脂肪细胞，提示高脂模型建立成功；经过间歇性低氧训练后，低氧组和低氧+肥胖组小鼠的平均体重及肝脏脂肪细胞分布密度和范围分别较对照组和肥胖组低，而血清瘦素水平明显增高；低氧+肥胖组小鼠肝脏瘦素受体的表达高于肥胖组。结果提示，适度的间歇性低氧可以通过提高血清瘦素水平和增强肝脏瘦素受体表达而使体重减轻，并有效防止肝细胞脂肪变。

关键词： 低氧; 体重; 瘦素; 瘦素受体

Effect of intermittent hypoxia on leptin and leptin receptor expression in obesity mice

Qin Ling, Song Zhi, Wen Sailan, Jing Ran, Li Cen, Xiang Yang, Qin Xiaoqun

A Seven-year Program Student, in Grade 2002, Clinical Medicine,Xiangya Medical School, Central South University.Changsha 410078,Hunan；China

Abstract

In order to explore the effect and underlying mechanism of hypoxia on body weight, the effect of intermittent moderate hypoxia on high-fat diet-induced obesity was observed in mice, and the role of leptin in hypoxic effect was identified. Healthy Kunming mice were divided randomly into 4 groups （n=20 in each group）. The control group： the mice were fed normally under the normal oxygen pressure. Hypoxia group： the mice were fed normally, and given intermittent moderate hypoxia training. Obesity group： the mice were fed diet rich in fat and sugar under the normal oxygen pressure. Hypoxia + obesity group： the mice were fed diet rich in fat and sugar, and given intermittent moderate hypoxia training. After 40 d of feeding and training, the body weight of mice was determined, and the average increasing rate of body weight in each group was calculated and normalized with food intake. Meanwhile, plasma leptin level was measured with ELISA method, and fatty degeneration and leptin receptor expression in liver were observed by Sudan Ⅲ staining and immunohistocbemistry, respectively. The obesity mouse model was successfully established with increases in body weight, plasma leptin level and distribution of adipocytes in the liver. The average body weight and density of adipocytes in the liver in hypoxia and hypoxia + obesity groups decreased obviously, while plasma leptin level and leptin receptor expression in the liver were increased. It is suggested that intermittent moderate hypoxia reduces body weight through elevating plasma leptin level and/or enhancing leptin receptor expression in the liver.

Key words: Hypoxia;Body weight;leptin;leptin receptor

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引用本文：

秦岭, 宋智, 文赛兰, 井然, 李岑, 向阳, 秦晓群. 间歇性低氧对肥胖小鼠瘦素及其受体表达的影响[J]. 生理学报 2007; 59 (3): 351-356.

Qin Ling, Song Zhi, Wen Sailan, Jing Ran, Li Cen, Xiang Yang, Qin Xiaoqun. Effect of intermittent hypoxia on leptin and leptin receptor expression in obesity mice. Acta Physiol Sin 2007; 59 (3): 351-356 (in Chinese with English abstract).